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DEG
DEG 15 contains 53,038 essential genes and 786 essential non-coding sequences in the following Organisms (Dec. 7, 2017).
Update for the BLAST service.

DEG 14.7 contains 53,038 essential genes and 786 essential non-coding sequences in the following Organisms (Oct. 24, 2016).
Add the essential genes in Escherichia coli ST131 strain EC958 based on the original article.
Organism (Prokaryotes) Essential genes Reference
Escherichia coli ST131 strain EC958 315 Phan, M.D., et al. (2013) The serum resistome of a globally disseminated multidrug resistant uropathogenic Escherichia coli clone, PLoS Genet., 9, e1003834. [PubMed]

DEG 14.6 contains 52,723 essential genes and 786 essential non-coding sequences in the following Organisms (Oct. 21, 2016).
Optimize DEG database and Fix a error in the search webpage.

DEG 14.5 contains 52,723 essential genes and 786 essential non-coding sequences in the following Organisms (Oct. 17, 2016).
Add the essential genes in Bacillus thuringiensis BMB171 based on the original article.
Organism (Prokaryotes) Essential genes Reference
Bacillus thuringiensis BMB171 516 Bishop, A.H., Rachwal, P.A. and Vaid, A. (2014) Identification of genes required by Bacillus thuringiensis for survival in soil by transposon-directed insertion site sequencing, Curr. Microbiol., 68, 477-485. [PubMed]

DEG 14.4 contains 52,207 essential genes and 786 essential non-coding sequences in the following Organisms (Oct. 13, 2016).
Add the essential genes in human K562 cells which are essential for Oxidative Phosphorylation based on the original article.
Organism (Eukaryotes) Essential genes Reference
Homo sapiens 191 Arroyo, J.D., et al. (2016) A Genome-wide CRISPR Death Screen Identifies Genes Essential for Oxidative Phosphorylation, Cell Metab. [PubMed]

DEG 14.3 contains 52,016 essential genes and 786 essential non-coding sequences in the following Organisms (Sept. 28, 2016).
Add the essential genes and non-coding elements in Agrobacterium fabrum str. C58 based on the original article.
Organism (Prokaryotes) Essential genes Reference
Brevundimonas subvibrioides ATCC 15264 412 Curtis, P.D. and Brun, Y.V. (2014) Identification of essential alphaproteobacterial genes reveals operational variability in conserved developmental and cell cycle systems, Mol. Microbiol., 93, 713-735. [PubMed]
Brevundimonas subvibrioides ATCC 15264 35 Curtis, P.D. and Brun, Y.V. (2014) Identification of essential alphaproteobacterial genes reveals operational variability in conserved developmental and cell cycle systems, Mol. Microbiol., 93, 713-735. [PubMed]

DEG 14.1 contains 51,604 essential genes and 752 essential non-coding sequences in the following Organisms (Sept. 27, 2016).
Add the essential genes and non-coding elements in Agrobacterium fabrum str. C58 based on the original article.
Organism (Prokaryotes) Essential genes Reference
Agrobacterium fabrum str. C58 361 Curtis, P.D. and Brun, Y.V. (2014) Identification of essential alphaproteobacterial genes reveals operational variability in conserved developmental and cell cycle systems, Mol. Microbiol., 93, 713-735. [PubMed]
Agrobacterium fabrum str. C58 11 Curtis, P.D. and Brun, Y.V. (2014) Identification of essential alphaproteobacterial genes reveals operational variability in conserved developmental and cell cycle systems, Mol. Microbiol., 93, 713-735. [PubMed]

DEG 13.9 contains 51,043 essential genes and 740 essential non-coding sequences in the following Organisms (Sept. 24, 2016).
Add the essential genes and non-coding elements in Acinetobacter baumannii ATCC 17978 with Murine model of pneumonia based on the original article.
Organism (Prokaryotes) Essential genes Reference
Acinetobacter baumannii ATCC 17978 156 Wang, N., et al. (2014) Genome-wide identification of Acinetobacter baumannii genes necessary for persistence in the lung, MBio, 5, e01163-01114. [PubMed]
Acinetobacter baumannii ATCC 17978 1 Wang, N., et al. (2014) Genome-wide identification of Acinetobacter baumannii genes necessary for persistence in the lung, MBio, 5, e01163-01114. [PubMed]

DEG 13.7 contains 50,887 essential genes and 739 essential non-coding sequences in the following Organisms (Sept. 23, 2016).
Add the essential genes and non-coding elements in Acinetobacter baumannii ATCC 17978 based on the original article.
Organism (Prokaryotes) Essential genes Reference
Acinetobacter baumannii ATCC 17978 458 Wang, N., et al. (2014) Genome-wide identification of Acinetobacter baumannii genes necessary for persistence in the lung, MBio, 5, e01163-01114. [PubMed]
Acinetobacter baumannii ATCC 17978 59 Wang, N., et al. (2014) Genome-wide identification of Acinetobacter baumannii genes necessary for persistence in the lung, MBio, 5, e01163-01114. [PubMed]

DEG 13.5 contains 50,429 essential genes and 646 essential non-coding sequences in the following Organisms (Sept. 20, 2016).
Add the essential genes in Streptococcus agalactiae A909 based on the original article.
Organism (Prokaryotes) Essential genes Reference
Streptococcus agalactiae A909 317 Hooven, T.A. et al. The Essential Genome of Streptococcus Agalactiae.BMC Genomics, 17, 406, 2016. [PubMed]

DEG 13.4 contains 50,112 essential genes and 646 essential non-coding sequences in the following Organisms (Sept. 19, 2016).
Add the essential genes in Mus musculus based on the original article.
Organism (Eukaryotes) Essential genes Reference
Mus musculus 410 Dickinson, M.E. et al. High-throughput discovery of novel developmental phenotypes. Nature (2016). [PubMed]

DEG 13.3 contains 49,702 essential genes and 646 essential non-coding sequences in the following Organisms (Jan. 7, 2016).
Update the orignal ids for part of essential genes in the information webpage.

DEG 13.2 contains 49,702 essential genes and 646 essential non-coding sequences in the following Organisms (Jan. 6, 2016).
Add the essential genes in Rhodopseudomonas palustris CGA009 based on the original article.
Organism (Prokaryotes) Essential genes Reference
Rhodopseudomonas palustris CGA009 522 Pechter, Kieran B., et al. "The essential genome of the metabolically versatile alphaproteobacterium Rhodopseudomonas palustris." Journal of bacteriology (2015): JB-00771. [PubMed]

DEG 13.1 contains 49,180 essential genes and 646 essential non-coding sequences in the following Organisms (Dec. 28, 2015).
Fix a error in the information webpage.

DEG 13.0contains 49,180 essential genes and 646 essential non-coding sequences in the following Organisms (Dec. 27, 2015).
Add the core essential genes in Homo sapiens in five cell-lines based on the original article.
Organism (Eukaryotes) Essential genes Type Reference
Homo sapiens 1196 A375 Hart, Traver, et al. "High-Resolution CRISPR Screens Reveal Fitness Genes and Genotype-Specific Cancer Liabilities." Cell (2015).[PubMed]
Homo sapiens 1892 DLD1 Hart, Traver, et al. "High-Resolution CRISPR Screens Reveal Fitness Genes and Genotype-Specific Cancer Liabilities." Cell (2015).[PubMed]
Homo sapiens 2196 GBM Hart, Traver, et al. "High-Resolution CRISPR Screens Reveal Fitness Genes and Genotype-Specific Cancer Liabilities." Cell (2015).[PubMed]
Homo sapiens 2070 HCT116 Hart, Traver, et al. "High-Resolution CRISPR Screens Reveal Fitness Genes and Genotype-Specific Cancer Liabilities." Cell (2015).[PubMed]
Homo sapiens 386 HCT116 by shRNA Hart, Traver, et al. "High-Resolution CRISPR Screens Reveal Fitness Genes and Genotype-Specific Cancer Liabilities." Cell (2015).[PubMed]
Homo sapiens 1694 HeLa Hart, Traver, et al. "High-Resolution CRISPR Screens Reveal Fitness Genes and Genotype-Specific Cancer Liabilities." Cell (2015).[PubMed]
Homo sapiens 2038 REP1 Hart, Traver, et al. "High-Resolution CRISPR Screens Reveal Fitness Genes and Genotype-Specific Cancer Liabilities." Cell (2015).[PubMed]

DEG 12.3 contains 37,738 essential genes and 646 essential non-coding sequences in the following Organisms (Dec. 21, 2015).
Add the essential genes in Homo sapiens Raji cells based on the original article.
Organism (Eukaryotes) Essential genes Reference
Homo sapiens 1461 Wang, Tim, et al. "Identification and characterization of essential genes in the human genome." Science 350.6264 (2015): 1096-1101. [PubMed]

DEG 12.2 contains 36,277 essential genes and 646 essential non-coding sequences in the following Organisms (Dec. 20, 2015).
Add the essential genes in Homo sapiens Jiyoye cells based on the original article.
Organism (Eukaryotes) Essential genes Reference
Homo sapiens 1627 Wang, Tim, et al. "Identification and characterization of essential genes in the human genome." Science 350.6264 (2015): 1096-1101. [PubMed]

DEG 12.1 contains 34,650 essential genes and 646 essential non-coding sequences in the following Organisms (Dec. 19, 2015).
Add the essential genes in Homo sapiens K562 cells based on the original article.
Organism (Eukaryotes) Essential genes Reference
Homo sapiens 1660 Wang, Tim, et al. "Identification and characterization of essential genes in the human genome." Science 350.6264 (2015): 1096-1101. [PubMed]

DEG 12.0 contains 32,990 essential genes and 646 essential non-coding sequences in the following Organisms (Dec. 18, 2015).
Add the essential genes in Homo sapiens KBM7 cells based on the original article.
Organism (Eukaryotes) Essential genes Reference
Homo sapiens 1877 Wang, Tim, et al. "Identification and characterization of essential genes in the human genome." Science 350.6264 (2015): 1096-1101. [PubMed]

DEG 11.9 contains 31,113 essential genes and 646 essential non-coding sequences in the following Organisms (Dec. 12, 2015).
Add the essential genes in Homo sapiens HAP1 cells based on the original article.
Organism (Eukaryotes) Essential genes Reference
Homo sapiens 2164 Blomen, Vincent A., et al. "Gene essentiality and synthetic lethality in haploid human cells." Science 350.6264 (2015): 1092-1096. [PubMed]

DEG 11.8 contains 28,949 essential genes and 646 essential non-coding sequences in the following Organisms (Dec. 11, 2015).
Add the essential genes in Homo sapiens KBM7 cells based on the original article.
Organism (Eukaryotes) Essential genes Reference
Homo sapiens 2038 Blomen, Vincent A., et al. "Gene essentiality and synthetic lethality in haploid human cells." Science 350.6264 (2015): 1092-1096. [PubMed]

DEG 11.7 contains 26,911 essential genes and 646 essential non-coding sequences in the following Organisms (Dec. 3, 2015).
Add the essential genes in Homo sapiens based on the original article.
Organism (Eukaryotes) Essential genes Reference
Homo sapiens 3137 Lek, Monkol, et al. "Analysis of protein-coding genetic variation in 60,706 humans." bioRxiv (2015): 030338. [PubMed]

DEG 11.6 contains 23,774 essential genes and 680 essential non-coding sequences in the following Organisms. (Dec. 2, 2015).
Fix a error in the information webpage.

DEG 11.5 contains 23,774 essential genes and 680 essential non-coding sequences in the following Organisms (Nov. 24, 2015).
Add the essential genes and RNAs in Synechococcus elongatus PCC 7942 based on the original article.
Organism (Eukaryotes) Essential genes Reference
Synechococcus elongatus PCC 7942 682 Rubin, Benjamin E., et al. "The essential gene set of a photosynthetic organism." Proceedings of the National Academy of Sciences (2015): 201519220. [PubMed]
Synechococcus elongatus PCC 7942 34 Rubin, Benjamin E., et al. "The essential gene set of a photosynthetic organism." Proceedings of the National Academy of Sciences (2015): 201519220. [PubMed]

DEG 11.4 contains 23,092 essential genes and 646 essential non-coding sequences in the following Organisms (Nov. 23, 2015).
Fix a error in the information webpage.

DEG 11.3 contains 23,092 essential genes and 646 essential non-coding sequences in the following Organisms (Oct. 8, 2015).
Add the essential genes in Porphyromonas gingivalis ATCC 33277 based on the original article.
Organism (Eukaryotes) Essential genes Reference
Porphyromonas gingivalis ATCC 33277 281 Hutcherson, Justin A., et al (2015). Comparison of inherently essential genes of Porphyromonas gingivalis identified in two transposon sequencing libraries. Molecular Oral Microbiology. [PubMed]

DEG 11.2 contains 22,811 essential genes and 646 essential non-coding sequences (July. 3, 2015).
Fix a bug in the BLAST web server.

DEG 11.1 contains 22,811 essential genes and 646 essential non-coding sequences (May. 30, 2015).
Add the essential genes in Streptococcus pyogenes MGAS5448 and NZ131 based on the original article.
Organism (Prokaryotes) Essential genes Reference
Streptococcus pyogenes MGAS5448 227 Le Breton Y. et al (2015). Essential Genes in the Core Genome of the Human Pathogen Streptococcus pyogenes. Sci. Rep. 5, 9838.[PubMed]
Streptococcus pyogenes NZ131 241

DEG 11.0 contains 22343 essential genes and 646 essential non-coding sequences (March. 21, 2015).
Add the essential genes in Pseudomonas aeruginosa PAO1 based on the original article.
Organism (Prokaryotes) Essential genes Reference
Pseudomonas aeruginosa PAO1 336 Turner, K.H., Wessel, A.K., Palmer, G.C., Murray, J.L. and Whiteley, M. (2015) Essential genome of Pseudomonas aeruginosa in cystic fibrosis sputum. Proc. Natl. Acad. Sci. U. S. A.[PubMed]

DEG 10.9 contains 22343 essential genes and 646 essential non-coding sequences in the following Organisms (Feb. 6, 2015).
Update the Uniprot ID and gene ontology annotation for the bacterial items.

DEG 10.7 contains 22343 essential genes and 646 essential non-coding sequences in the following Organisms (Jan. 13, 2015).
Update COG numbers into DEG records.

DEG 10.6 contains 22343 essential genes and 646 essential non-coding sequences in the following Organisms (Sept. 11, 2014).
Fix a bug in the BLAST webpage.

DEG 10.5 contains 22343 essential genes and 646 essential non-coding sequences in the following Organisms (Sept. 6, 2014).
Rorganize the essential genes in Danio rerio based on the original article.
Organism (Eukaryotes) Essential genes Reference
Danio rerio 315 Amsterdam A, et al (2004). Identification of 315 genes essential for early zebrafish development. Proc Natl Acad Sci U S A, 101:12792-7. [PubMed]

DEG 10.4 contains 22316 essential genes and 646 essential non-coding sequences in the following Organisms (Jul. 25, 2014).
Fix a bug to support the latest GenBank format.

DEG 10.3 contains 22316 essential genes and 646 essential non-coding sequences in the following Organisms (Jul. 24, 2014).
Organism (Prokaryotes) Essential genes Reference
Burkholderia pseudomallei K96243 505 Moule, M.G., et al. (2014) Genome-Wide Saturation Mutagenesis of Burkholderia pseudomallei K96243 Predicts Essential Genes and Novel Targets for Antimicrobial Development. MBio 5, e00926-00913 [PubMed]

DEG 10.2 contains 21811 essential genes and 646 essential non-coding sequences in the following Organisms (Jul. 12, 2014).
Update the GI numbers of Bacillus subtilis 168 and Escherichia coli MG1655 II.

DEG 10.1 contains 21811 essential genes and 646 essential non-coding sequences in the following Organisms (Jun. 6, 2014).
Organism (Prokaryotes) Essential genes Reference
Bacteroides fragilis 638R 547 Yaligara Veeranagouda, et al (2014). "Identification of genes required for the survival of B. fragilis using massive parallel sequencing of a saturated transposon mutant library". BMC Genomics, 15:429.PubMed]

 

DEG 10.0 contains 21264 essential genes and 646 essential non-coding sequences in the following Organisms (Sep. 16, 2013).
No Organism (Prokaryotes) Essential genes Reference
1 Acinetobacter baylyi ADP1 de Berardinis V, et al (2008). A complete collection of single-gene deletion mutants of Acinetobacter baylyi ADP1. Mol Syst Biol. [PubMed]
2 Bacillus subtilis 168 Kobayashi K, et al (2003). Essential Bacillus subtilis genes. Proc Natl Acad Sci U S A, 100:4678-83. [PubMed]
3 Bacteroides thetaiotaomicron VPI-5482 Goodman Al, et al (2009). Identifying Genetic Determinants Needed to Establish a Human Gut Symbiont in Its Habitat. Cell Host & Microbe, 6: 279-289. [PubMed]
4 Burkholderia thailandensis E264 Baugh, Loren, et al. "Combining Functional and Structural Genomics to Sample the Essential Burkholderia Structome." PloS one 8.1 (2013): e53851. [PubMed]
5 Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819 Metris, Aline, et al. "In vivo and in silico determination of essential genes of Campylobacter jejuni." BMC genomics 12.1 (2011): 535. [PubMed]
6 Caulobacter crescentus Christen B, et al (2011). The essential genome of a bacterium. Mol Syst Biol, 7:528. [PubMed]
7 Escherichia coli MG1655 I Gerdes SY, et al (2003). Experimental determination and system level analysis of essential genes in Escherichia coli MG1655. J Bacteriol, 185:5673-84. [PubMed]
8 Escherichia coli MG1655 II Baba T, et al (2006). Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol. [PubMed]
9 Francisella novicida U112 Gallagher LA, et al (2007). A comprehensive transposon mutant library of Francisella novicida, a bioweapon surrogate. Proc Natl Acad Sci U S A, 104:1009-14. [PubMed]
10 Haemophilus influenzae Rd KW20 Akerley BJ, et al (2002). A genome-scale analysis for identification of genes required for growth or survival of Haemophilus influenzae. Proc Natl Acad Sci U S A, 99:966-71. [PubMed]
11 Helicobacter pylori 26695 Salama NR, et al (2004). Global transposon mutagenesis and essential gene analysis of Helicobacter pylori. J Bacteriol, 186:7926-35. [PubMed]
12 Mycobacterium tuberculosis H37Rv Sassetti CM, et al (2003). Genes required for mycobacterial growth defined by high density mutagenesis. Mol Microbiol, 48:77-84. [PubMed]
13 Mycobacterium tuberculosis H37Rv II Griffin, Jennifer E., et al. "High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism." PLoS pathogens 7.9 (2011): e1002251. [PubMed]
14 Mycobacterium tuberculosis H37Rv III Zhang, Yanjia J., et al. "Global assessment of genomic regions required for growth in Mycobacterium tuberculosis." PLoS pathogens 8.9 (2012): e1002946. [PubMed]
15 Mycoplasma genitalium G37 Glass JI, et al (2006). Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A, 103:425-30. [PubMed]
16 Mycoplasma pulmonis UAB CTIP French CT, et al (2008). Large-scale transposon mutagenesis of Mycoplasma pulmonis. Mol Microbiol. [PubMed]
17 Porphyromonas gingivalis ATCC 33277 Klein BA, et al (2012). Identification of essential genes of the periodontal pathogen Porphyromonas. BMC Genomics, 13: 578. [PubMed]
18 Pseudomonas aeruginosa PAO1 Gallagher, Larry A., Jay Shendure, and Colin Manoil. "Genome-scale identification of resistance functions in Pseudomonas aeruginosa using Tn-seq." MBio 2.1 (2011). [PubMed]
19 Pseudomonas aeruginosa UCBPP-PA14 Liberati NT, et al (2006). An ordered, nonredundant library of Pseudomonas aeruginosa strain PA14 transposon insertion mutants. Proc Natl Acad Sci U S A, 103:2833-8. [PubMed]
20 Salmonella enterica serovar Typhi Langridge GC, et al (2009). Simultaneous assay of every Salmonella Typhi gene using one million transposon mutants. Genome Res, 19:2308-16. [PubMed]
21 Salmonella enterica serovar Typhi Ty2 Barquist, Lars, et al. "A comparison of dense transposon insertion libraries in the Salmonella serovars Typhi and Typhimurium." Nucleic acids research 41.8 (2013): 4549-4564. [PubMed]
22 Salmonella enterica serovar Typhimurium SL1344 Barquist, Lars, et al. "A comparison of dense transposon insertion libraries in the Salmonella serovars Typhi and Typhimurium." Nucleic acids research 41.8 (2013): 4549-4564. [PubMed]
23 Salmonella enterica subsp. enterica serovar Typhimurium str. 14028S Khatiwara, Anita, et al. "Genome scanning for conditionally essential genes in salmonella enterica serotype typhimurium." Applied and environmental microbiology 78.9 (2012): 3098-3107. [PubMed]
24 Salmonella typhimurium LT2 Knuth K, et al (2004). Large-scale identification of essential Salmonella genes by trapping lethal insertions. Mol Microbiol, 51:1729-44. [PubMed]
25 Shewanella oneidensis MR-1 Deutschbauer, Adam, et al. "Evidence-based annotation of gene function in Shewanella oneidensis MR-1 using genome-wide fitness profiling across 121 conditions." PLoS genetics 7.11 (2011): e1002385. [PubMed]
26 Sphingomonas wittichii RW1 Roggo, Clemence, et al. "Genome-wide transposon insertion scanning of environmental survival functions in the polycyclic aromatic hydrocarbon degrading bacterium Sphingomonas wittichii RW1." Environmental microbiology (2013). [PubMed]
27 Staphylococcus aureus N315 Ji Y, et al (2001). Identification of critical staphylococcal genes using conditional phenotypes generated by antisense RNA. Science, 293:2266-9. [PubMed]

Forsyth RA, et al (2002). A genome-wide strategy for the identification of essential genes in Staphylococcus aureus. Mol Microbiol, 43:1387-400. [PubMed]
28 Staphylococcus aureus NCTC 8325 Chaudhuri RR, et al (2009). Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH). BMC Genomics, 10:291. [PubMed]
39 Streptococcus pneumoniae Thanassi JA, et al (2002). Identification of 113 conserved essential genes using a high-throughput gene disruption system in Streptococcus pneumoniae. Nucleic Acids Res, 30:3152-62. [PubMed]

Song JH, et al (2005). Identification of Essential Genes in Streptococcus pneumoniae by Allelic Replacement Mutagenesis. Mol Cells, 19:365-74. [PubMed]
30 Streptococcus sanguinis Xu, P et al(2011). Genome-wide essential gene identification in Streptococcus sanguinis. Sci. Rep. 1, 125; DOI:10.1038/srep00125. [PubMed]
31 Vibrio cholerae N16961 Cameron DE, Urbach MJ,Mekalanos JJ(2008). A defined transposon mutant library and its use in identifying motility genes in Vibrio cholerae. PNAS, 105(25):8736-8741. [PubMed]
No Organism (Eukaryotes) Essential genes Reference
1 Arabidopsis thaliana Meinke D, et al(2008). Identifying essential genes in Arabidopsis thaliana. Trends Plant Sci, 13:483-91. [PubMed]
2 Aspergillus fumigatus Hu W, et al (2007). Essential gene identification and drug target prioritization in Aspergillus fumigatus. PLoS Pathog, 3:e24. [PubMed]
3 Caenorhabditis elegans Kamath RS, et al (2003). Systematic functional analysis of the Caenorhabditis elegans genome using RNAi. Nature, 421:231-7. [PubMed]
4 Danio rerio Amsterdam A, et al (2004). Identification of 315 genes essential for early zebrafish development. Proc Natl Acad Sci U S A, 101:12792-7. [PubMed]
5 Drosophila melanogaster Spradling AC, et al (1999). The Berkeley Drosophila Genome Project gene disruption project: Single P-element insertions mutating 25% of vital Drosophila genes. Genetics, 153:135-77. [PubMed]
6 Homo sapiens Liao BY, Zhang J (2008). Null mutations in human and mouse orthologs frequently result in different phenotypes. Proc Natl Acad Sci U S A, 105:6987-92. [PubMed]
7 Homo sapiens I Georgi, Benjamin, Benjamin F. Voight, and Maja Bucan. "From Mouse to Human: Evolutionary Genomics Analysis of Human Orthologs of Essential Genes." PLoS genetics 9.5 (2013): e1003484. [PubMed]
8 Mus musculus Liao BY, Zhang J (2007). Mouse duplicate genes are as essential as singletons. Trends Genet, 23:378-81. [PubMed]
9 Saccharomyces cerevisiae Giaever G, et al (2002). Functional profiling of the Saccharomyces cerevisiae genome. Nature, 418:387-91. [PubMed]
10 Schizosaccharomyces pombe 972h- Kim, Dong-Uk, et al. "Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe." Nature biotechnology 28.6 (2010): 617-623. [PubMed]
No Organism (Archaea) Essential genes Reference
1 Methanococcus maripaludis S2 Sarmiento F, et al (2013). Genome-scale analysis of gene function in the hydrogenotrophic methanogenic archaeon Methanococcus maripaludis. Proc Natl Acad Sci U S A, 110:4726-31. [PubMed]
No Organism (Non-coding) Essential sequences Reference
1 Caulobacter crescentus Christen B, et al (2011). The essential genome of a bacterium. Mol Syst Biol, 7:528. [PubMed]
2 Mycobacterium tuberculosis H37Rv III Zhang, Yanjia J., et al. "Global assessment of genomic regions required for growth in Mycobacterium tuberculosis." PLoS pathogens 8.9 (2012): e1002946. [PubMed]
3 Salmonella enterica serovar Typhi Ty2 Barquist, Lars, et al. "A comparison of dense transposon insertion libraries in the Salmonella serovars Typhi and Typhimurium." Nucleic acids research 41.8 (2013): 4549-4564. [PubMed]
4 Salmonella enterica serovar Typhimurium SL1344 Barquist, Lars, et al. "A comparison of dense transposon insertion libraries in the Salmonella serovars Typhi and Typhimurium." Nucleic acids research 41.8 (2013): 4549-4564. [PubMed]
5 Sphingomonas wittichii RW1 Roggo, Clemence, et al. "Genome-wide transposon insertion scanning of environmental survival functions in the polycyclic aromatic hydrocarbon degrading bacterium Sphingomonas wittichii RW1." Environmental microbiology (2013). [PubMed]

 

DEG 9.0 contains 20894 essential genes and 599 essential non-coding sequences in the following Organisms (Aug. 26, 2013).
No Organism (Prokaryotes) Essential genes Reference
1 Acinetobacter baylyi ADP1 de Berardinis V, et al (2008). A complete collection of single-gene deletion mutants of Acinetobacter baylyi ADP1. Mol Syst Biol. [PubMed]
2 Bacillus subtilis 168 Kobayashi K, et al (2003). Essential Bacillus subtilis genes. Proc Natl Acad Sci U S A, 100:4678-83. [PubMed]
3 Bacteroides thetaiotaomicron VPI-5482 Goodman Al, et al (2009). Identifying Genetic Determinants Needed to Establish a Human Gut Symbiont in Its Habitat. Cell Host & Microbe, 6: 279-289. [PubMed]
4 Burkholderia thailandensis E264 Baugh, Loren, et al. "Combining Functional and Structural Genomics to Sample the Essential Burkholderia Structome." PloS one 8.1 (2013): e53851. [PubMed]
5 Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819 Metris, Aline, et al. "In vivo and in silico determination of essential genes of Campylobacter jejuni." BMC genomics 12.1 (2011): 535. [PubMed]
6 Caulobacter crescentus Christen B, et al (2011). The essential genome of a bacterium. Mol Syst Biol, 7:528. [PubMed]
7 Escherichia coli MG1655 Gerdes SY, et al (2003). Experimental determination and system level analysis of essential genes in Escherichia coli MG1655. J Bacteriol, 185:5673-84. [PubMed]

Baba T, et al (2006). Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol. [PubMed]
8 Escherichia coli MG1655 I Gerdes SY, et al (2003). Experimental determination and system level analysis of essential genes in Escherichia coli MG1655. J Bacteriol, 185:5673-84. [PubMed]
9 Escherichia coli MG1655 II Baba T, et al (2006). Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol. [PubMed]
10 Francisella novicida U112 Gallagher LA, et al (2007). A comprehensive transposon mutant library of Francisella novicida, a bioweapon surrogate. Proc Natl Acad Sci U S A, 104:1009-14. [PubMed]
11 Haemophilus influenzae Rd KW20 Akerley BJ, et al (2002). A genome-scale analysis for identification of genes required for growth or survival of Haemophilus influenzae. Proc Natl Acad Sci U S A, 99:966-71. [PubMed]
12 Helicobacter pylori 26695 Salama NR, et al (2004). Global transposon mutagenesis and essential gene analysis of Helicobacter pylori. J Bacteriol, 186:7926-35. [PubMed]
13 Mycobacterium tuberculosis H37Rv Sassetti CM, et al (2003). Genes required for mycobacterial growth defined by high density mutagenesis. Mol Microbiol, 48:77-84. [PubMed]
14 Mycobacterium tuberculosis H37Rv II Griffin, Jennifer E., et al. "High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism." PLoS pathogens 7.9 (2011): e1002251. [PubMed]
15 Mycobacterium tuberculosis H37Rv III Zhang, Yanjia J., et al. "Global assessment of genomic regions required for growth in Mycobacterium tuberculosis." PLoS pathogens 8.9 (2012): e1002946. [PubMed]
16 Mycoplasma genitalium G37 Glass JI, et al (2006). Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A, 103:425-30. [PubMed]
17 Mycoplasma pulmonis UAB CTIP French CT, et al (2008). Large-scale transposon mutagenesis of Mycoplasma pulmonis. Mol Microbiol. [PubMed]
18 Porphyromonas gingivalis ATCC 33277 Klein BA, et al (2012). Identification of essential genes of the periodontal pathogen Porphyromonas. BMC Genomics, 13: 578. [PubMed]
19 Pseudomonas aeruginosa PAO1 Gallagher, Larry A., Jay Shendure, and Colin Manoil. "Genome-scale identification of resistance functions in Pseudomonas aeruginosa using Tn-seq." MBio 2.1 (2011). [PubMed]
20 Pseudomonas aeruginosa UCBPP-PA14 Liberati NT, et al (2006). An ordered, nonredundant library of Pseudomonas aeruginosa strain PA14 transposon insertion mutants. Proc Natl Acad Sci U S A, 103:2833-8. [PubMed]
21 Salmonella enterica serovar Typhi Langridge GC, et al (2009). Simultaneous assay of every Salmonella Typhi gene using one million transposon mutants. Genome Res, 19:2308-16. [PubMed]
22 Salmonella enterica subsp. enterica serovar Typhimurium str. 14028S Khatiwara, Anita, et al. "Genome scanning for conditionally essential genes in salmonella enterica serotype typhimurium." Applied and environmental microbiology 78.9 (2012): 3098-3107. [PubMed]
23 Salmonella typhimurium LT2 Knuth K, et al (2004). Large-scale identification of essential Salmonella genes by trapping lethal insertions. Mol Microbiol, 51:1729-44. [PubMed]
24 Shewanella oneidensis MR-1 Deutschbauer, Adam, et al. "Evidence-based annotation of gene function in Shewanella oneidensis MR-1 using genome-wide fitness profiling across 121 conditions." PLoS genetics 7.11 (2011): e1002385. [PubMed]
25 Sphingomonas wittichii RW1 Roggo, Clemence, et al. "Genome-wide transposon insertion scanning of environmental survival functions in the polycyclic aromatic hydrocarbon degrading bacterium Sphingomonas wittichii RW1." Environmental microbiology (2013). [PubMed]
26 Staphylococcus aureus N315 Ji Y, et al (2001). Identification of critical staphylococcal genes using conditional phenotypes generated by antisense RNA. Science, 293:2266-9. [PubMed]

Forsyth RA, et al (2002). A genome-wide strategy for the identification of essential genes in Staphylococcus aureus. Mol Microbiol, 43:1387-400. [PubMed]
27 Staphylococcus aureus NCTC 8325 Chaudhuri RR, et al (2009). Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH). BMC Genomics, 10:291. [PubMed]
28 Streptococcus pneumoniae Thanassi JA, et al (2002). Identification of 113 conserved essential genes using a high-throughput gene disruption system in Streptococcus pneumoniae. Nucleic Acids Res, 30:3152-62. [PubMed]

Song JH, et al (2005). Identification of Essential Genes in Streptococcus pneumoniae by Allelic Replacement Mutagenesis. Mol Cells, 19:365-74. [PubMed]
29 Streptococcus sanguinis Xu, P et al(2011). Genome-wide essential gene identification in Streptococcus sanguinis. Sci. Rep. 1, 125; DOI:10.1038/srep00125. [PubMed]
30 Vibrio cholerae N16961 Cameron DE, Urbach MJ,Mekalanos JJ(2008). A defined transposon mutant library and its use in identifying motility genes in Vibrio cholerae. PNAS, 105(25):8736-8741. [PubMed]
No Organism (Eukaryotes) Essential genes Reference
1 Arabidopsis thaliana Meinke D, et al(2008). Identifying essential genes in Arabidopsis thaliana. Trends Plant Sci, 13:483-91. [PubMed]
2 Aspergillus fumigatus Hu W, et al (2007). Essential gene identification and drug target prioritization in Aspergillus fumigatus. PLoS Pathog, 3:e24. [PubMed]
3 Caenorhabditis elegans Kamath RS, et al (2003). Systematic functional analysis of the Caenorhabditis elegans genome using RNAi. Nature, 421:231-7. [PubMed]
4 Danio rerio Amsterdam A, et al (2004). Identification of 315 genes essential for early zebrafish development. Proc Natl Acad Sci U S A, 101:12792-7. [PubMed]
5 Drosophila melanogaster Spradling AC, et al (1999). The Berkeley Drosophila Genome Project gene disruption project: Single P-element insertions mutating 25% of vital Drosophila genes. Genetics, 153:135-77. [PubMed]
6 Homo sapiens Liao BY, Zhang J (2008). Null mutations in human and mouse orthologs frequently result in different phenotypes. Proc Natl Acad Sci U S A, 105:6987-92. [PubMed]
7 Homo sapiens I Georgi, Benjamin, Benjamin F. Voight, and Maja Bucan. "From Mouse to Human: Evolutionary Genomics Analysis of Human Orthologs of Essential Genes." PLoS genetics 9.5 (2013): e1003484. [PubMed]
8 Mus musculus Liao BY, Zhang J (2007). Mouse duplicate genes are as essential as singletons. Trends Genet, 23:378-81. [PubMed]
9 Saccharomyces cerevisiae Giaever G, et al (2002). Functional profiling of the Saccharomyces cerevisiae genome. Nature, 418:387-91. [PubMed]
10 Schizosaccharomyces pombe 972h- Kim, Dong-Uk, et al. "Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe." Nature biotechnology 28.6 (2010): 617-623. [PubMed]
No Organism (Archaea) Essential genes Reference
1 Methanococcus maripaludis S2 Sarmiento F, et al (2013). Genome-scale analysis of gene function in the hydrogenotrophic methanogenic archaeon Methanococcus maripaludis. Proc Natl Acad Sci U S A, 110:4726-31. [PubMed]
No Organism (Non-coding) Essential sequences Reference
1 Caulobacter crescentus Christen B, et al (2011). The essential genome of a bacterium. Mol Syst Biol, 7:528. [PubMed]
2 Mycobacterium tuberculosis H37Rv III Zhang, Yanjia J., et al. "Global assessment of genomic regions required for growth in Mycobacterium tuberculosis." PLoS pathogens 8.9 (2012): e1002946. [PubMed]
3 Sphingomonas wittichii RW1 Roggo, Clemence, et al. "Genome-wide transposon insertion scanning of environmental survival functions in the polycyclic aromatic hydrocarbon degrading bacterium Sphingomonas wittichii RW1." Environmental microbiology (2013). [PubMed]

 

DEG 8.5 contains 15900 essential genes in the following Organisms ( July 6,2013).
No Organism (Prokaryotes) Essential genes Reference
1 Acinetobacter baylyi ADP1 de Berardinis V, et al (2008). A complete collection of single-gene deletion mutants of Acinetobacter baylyi ADP1. Mol Syst Biol. [PubMed]
2 Bacillus subtilis 168 Kobayashi K, et al (2003). Essential Bacillus subtilis genes. Proc Natl Acad Sci U S A, 100:4678-83. [PubMed]
3 Bacteroides thetaiotaomicron VPI-5482 Goodman Al, et al (2009). Identifying Genetic Determinants Needed to Establish a Human Gut Symbiont in Its Habitat. Cell Host & Microbe, 6: 279-289. [PubMed]
4 Burkholderia thailandensis E264 Baugh, Loren, et al. "Combining Functional and Structural Genomics to Sample the Essential Burkholderia Structome." PloS one 8.1 (2013): e53851. [PubMed]
5 Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819 Metris, Aline, et al. "In vivo and in silico determination of essential genes of Campylobacter jejuni." BMC genomics 12.1 (2011): 535. [PubMed]
6 Caulobacter crescentus Christen B, et al (2011). The essential genome of a bacterium. Mol Syst Biol, 7:528. [PubMed]
7 Escherichia coli MG1655 Gerdes SY, et al (2003). Experimental determination and system level analysis of essential genes in Escherichia coli MG1655. J Bacteriol, 185:5673-84. [PubMed]

Baba T, et al (2006). Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol. [PubMed]
8 Escherichia coli MG1655 I Gerdes SY, et al (2003). Experimental determination and system level analysis of essential genes in Escherichia coli MG1655. J Bacteriol, 185:5673-84. [PubMed]
9 Escherichia coli MG1655 II Baba T, et al (2006). Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol. [PubMed]
10 Francisella novicida U112 Gallagher LA, et al (2007). A comprehensive transposon mutant library of Francisella novicida, a bioweapon surrogate. Proc Natl Acad Sci U S A, 104:1009-14. [PubMed]
11 Haemophilus influenzae Rd KW20 Akerley BJ, et al (2002). A genome-scale analysis for identification of genes required for growth or survival of Haemophilus influenzae. Proc Natl Acad Sci U S A, 99:966-71. [PubMed]
12 Helicobacter pylori 26695 Salama NR, et al (2004). Global transposon mutagenesis and essential gene analysis of Helicobacter pylori. J Bacteriol, 186:7926-35. [PubMed]
13 Mycobacterium tuberculosis H37Rv Sassetti CM, et al (2003). Genes required for mycobacterial growth defined by high density mutagenesis. Mol Microbiol, 48:77-84. [PubMed]
14 Mycobacterium tuberculosis H37Rv II Griffin, Jennifer E., et al. "High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism." PLoS pathogens 7.9 (2011): e1002251. [PubMed]
15 Mycobacterium tuberculosis H37Rv III Zhang, Yanjia J., et al. "Global assessment of genomic regions required for growth in Mycobacterium tuberculosis." PLoS pathogens 8.9 (2012): e1002946. [PubMed]
16 Mycoplasma genitalium G37 Glass JI, et al (2006). Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A, 103:425-30. [PubMed]
17 Mycoplasma pulmonis UAB CTIP French CT, et al (2008). Large-scale transposon mutagenesis of Mycoplasma pulmonis. Mol Microbiol. [PubMed]
18 Porphyromonas gingivalis ATCC 33277 Klein BA, et al (2012). Identification of essential genes of the periodontal pathogen Porphyromonas. BMC Genomics, 13: 578. [PubMed]
19 Pseudomonas aeruginosa PAO1 Gallagher, Larry A., Jay Shendure, and Colin Manoil. "Genome-scale identification of resistance functions in Pseudomonas aeruginosa using Tn-seq." MBio 2.1 (2011). [PubMed]
20 Pseudomonas aeruginosa UCBPP-PA14 Liberati NT, et al (2006). An ordered, nonredundant library of Pseudomonas aeruginosa strain PA14 transposon insertion mutants. Proc Natl Acad Sci U S A, 103:2833-8. [PubMed]
21 Salmonella enterica serovar Typhi Langridge GC, et al (2009). Simultaneous assay of every Salmonella Typhi gene using one million transposon mutants. Genome Res, 19:2308-16. [PubMed]
22 Salmonella enterica subsp. enterica serovar Typhimurium str. 14028S Khatiwara, Anita, et al. "Genome scanning for conditionally essential genes in salmonella enterica serotype typhimurium." Applied and environmental microbiology 78.9 (2012): 3098-3107. [PubMed]
23 Salmonella typhimurium LT2 Knuth K, et al (2004). Large-scale identification of essential Salmonella genes by trapping lethal insertions. Mol Microbiol, 51:1729-44. [PubMed]
24 Shewanella oneidensis MR-1 Deutschbauer, Adam, et al. "Evidence-based annotation of gene function in Shewanella oneidensis MR-1 using genome-wide fitness profiling across 121 conditions." PLoS genetics 7.11 (2011): e1002385. [PubMed]
25 Sphingomonas wittichii RW1 Roggo, Clemence, et al. "Genome-wide transposon insertion scanning of environmental survival functions in the polycyclic aromatic hydrocarbon degrading bacterium Sphingomonas wittichii RW1." Environmental microbiology (2013). [PubMed]
26 Staphylococcus aureus N315 Ji Y, et al (2001). Identification of critical staphylococcal genes using conditional phenotypes generated by antisense RNA. Science, 293:2266-9. [PubMed]

Forsyth RA, et al (2002). A genome-wide strategy for the identification of essential genes in Staphylococcus aureus. Mol Microbiol, 43:1387-400. [PubMed]
27 Staphylococcus aureus NCTC 8325 Chaudhuri RR, et al (2009). Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH). BMC Genomics, 10:291. [PubMed]
28 Streptococcus pneumoniae Thanassi JA, et al (2002). Identification of 113 conserved essential genes using a high-throughput gene disruption system in Streptococcus pneumoniae. Nucleic Acids Res, 30:3152-62. [PubMed]

Song JH, et al (2005). Identification of Essential Genes in Streptococcus pneumoniae by Allelic Replacement Mutagenesis. Mol Cells, 19:365-74. [PubMed]
29 Streptococcus sanguinis Xu, P et al(2011). Genome-wide essential gene identification in Streptococcus sanguinis. Sci. Rep. 1, 125; DOI:10.1038/srep00125. [PubMed]
30 Vibrio cholerae N16961 Cameron DE, Urbach MJ,Mekalanos JJ(2008). A defined transposon mutant library and its use in identifying motility genes in Vibrio cholerae. PNAS, 105(25):8736-8741. [PubMed]
No Organism (Eukaryotes) Essential genes Reference
1 Arabidopsis thaliana Meinke D, et al(2008). Identifying essential genes in Arabidopsis thaliana. Trends Plant Sci, 13:483-91. [PubMed]
2 Aspergillus fumigatus Hu W, et al (2007). Essential gene identification and drug target prioritization in Aspergillus fumigatus. PLoS Pathog, 3:e24. [PubMed]
3 Caenorhabditis elegans Kamath RS, et al (2003). Systematic functional analysis of the Caenorhabditis elegans genome using RNAi. Nature, 421:231-7. [PubMed]
4 Danio rerio Amsterdam A, et al (2004). Identification of 315 genes essential for early zebrafish development. Proc Natl Acad Sci U S A, 101:12792-7. [PubMed]
5 Drosophila melanogaster Spradling AC, et al (1999). The Berkeley Drosophila Genome Project gene disruption project: Single P-element insertions mutating 25% of vital Drosophila genes. Genetics, 153:135-77. [PubMed]
6 Homo sapiens Liao BY, Zhang J (2008). Null mutations in human and mouse orthologs frequently result in different phenotypes. Proc Natl Acad Sci U S A, 105:6987-92. [PubMed]
7 Homo sapiens I Georgi, Benjamin, Benjamin F. Voight, and Maja Bucan. "From Mouse to Human: Evolutionary Genomics Analysis of Human Orthologs of Essential Genes." PLoS genetics 9.5 (2013): e1003484. [PubMed]
8 Mus musculus Liao BY, Zhang J (2007). Mouse duplicate genes are as essential as singletons. Trends Genet, 23:378-81. [PubMed]
9 Saccharomyces cerevisiae Giaever G, et al (2002). Functional profiling of the Saccharomyces cerevisiae genome. Nature, 418:387-91. [PubMed]
10 Schizosaccharomyces pombe 972h- Kim, Dong-Uk, et al. "Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe." Nature biotechnology 28.6 (2010): 617-623. [PubMed]
No Organism (Archaea) Essential genes Reference
1 Methanococcus maripaludis S2 Sarmiento F, et al (2013). Genome-scale analysis of gene function in the hydrogenotrophic methanogenic archaeon Methanococcus maripaludis. Proc Natl Acad Sci U S A, 110:4726-31. [PubMed]

 

DEG 8.0 contains 15584 essential genes in the following Organisms ( July 5,2013).
No Organism (Prokaryotes) Essential genes Reference
1 Acinetobacter baylyi ADP1 de Berardinis V, et al (2008). A complete collection of single-gene deletion mutants of Acinetobacter baylyi ADP1. Mol Syst Biol. [PubMed]
2 Bacillus subtilis 168 Kobayashi K, et al (2003). Essential Bacillus subtilis genes. Proc Natl Acad Sci U S A, 100:4678-83. [PubMed]
3 Bacteroides thetaiotaomicron VPI-5482 Goodman Al, et al (2009). Identifying Genetic Determinants Needed to Establish a Human Gut Symbiont in Its Habitat. Cell Host & Microbe, 6: 279¨C289. [PubMed]
4 Burkholderia thailandensis E264 Baugh, Loren, et al. "Combining Functional and Structural Genomics to Sample the Essential Burkholderia Structome." PloS one 8.1 (2013): e53851. [PubMed]
5 Caulobacter crescentus Christen B, et al (2011). The essential genome of a bacterium. Mol Syst Biol, 7:528. [PubMed]
6 Escherichia coli MG1655 Gerdes SY, et al (2003). Experimental determination and system level analysis of essential genes in Escherichia coli MG1655. J Bacteriol, 185:5673-84. [PubMed]

Baba T, et al (2006). Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol. [PubMed]
7 Escherichia coli MG1655 I Gerdes SY, et al (2003). Experimental determination and system level analysis of essential genes in Escherichia coli MG1655. J Bacteriol, 185:5673-84. [PubMed]
8 Escherichia coli MG1655 II Baba T, et al (2006). Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol. [PubMed]
9 Francisella novicida U112 Gallagher LA, et al (2007). A comprehensive transposon mutant library of Francisella novicida, a bioweapon surrogate. Proc Natl Acad Sci U S A, 104:1009-14. [PubMed]
10 Haemophilus influenzae Rd KW20 Akerley BJ, et al (2002). A genome-scale analysis for identification of genes required for growth or survival of Haemophilus influenzae. Proc Natl Acad Sci U S A, 99:966-71. [PubMed]
11 Helicobacter pylori 26695 Salama NR, et al (2004). Global transposon mutagenesis and essential gene analysis of Helicobacter pylori. J Bacteriol, 186:7926-35. [PubMed]
12 Mycobacterium tuberculosis H37Rv Sassetti CM, et al (2003). Genes required for mycobacterial growth defined by high density mutagenesis. Mol Microbiol, 48:77-84. [PubMed]
13 Mycobacterium tuberculosis H37Rv II Griffin, Jennifer E., et al. "High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism." PLoS pathogens 7.9 (2011): e1002251. [PubMed]
14 Mycoplasma genitalium G37 Glass JI, et al (2006). Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A, 103:425-30. [PubMed]
15 Mycoplasma pulmonis UAB CTIP French CT, et al (2008). Large-scale transposon mutagenesis of Mycoplasma pulmonis. Mol Microbiol. [PubMed]
16 Porphyromonas gingivalis ATCC 33277 Klein BA, et al (2012). Identification of essential genes of the periodontal pathogen Porphyromonas. BMC Genomics, 13: 578. [PubMed]
17 Pseudomonas aeruginosa UCBPP-PA14 Liberati NT, et al (2006). An ordered, nonredundant library of Pseudomonas aeruginosa strain PA14 transposon insertion mutants. Proc Natl Acad Sci U S A, 103:2833-8. [PubMed]
18 Salmonella enterica serovar Typhi Langridge GC, et al (2009). Simultaneous assay of every Salmonella Typhi gene using one million transposon mutants. Genome Res, 19:2308-16. [PubMed]
19 Salmonella enterica subsp. enterica serovar Typhimurium str. 14028S Khatiwara, Anita, et al. "Genome scanning for conditionally essential genes in salmonella enterica serotype typhimurium." Applied and environmental microbiology 78.9 (2012): 3098-3107. [PubMed]
20 Salmonella typhimurium LT2 Knuth K, et al (2004). Large-scale identification of essential Salmonella genes by trapping lethal insertions. Mol Microbiol, 51:1729-44. [PubMed]
21 Staphylococcus aureus N315 Ji Y, et al (2001). Identification of critical staphylococcal genes using conditional phenotypes generated by antisense RNA. Science, 293:2266-9. [PubMed]

Forsyth RA, et al (2002). A genome-wide strategy for the identification of essential genes in Staphylococcus aureus. Mol Microbiol, 43:1387-400. [PubMed]

Ko KS, et al (2006). Screening of Essential Genes in Staphylococcus aureus N315 Using Comparative Genomics and Allelic Replacement Mutagenesis. J Microbiol Biotechnol, 16:623-32.
22 Staphylococcus aureus NCTC 8325 Chaudhuri RR, et al (2009). Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH). BMC Genomics, 10:291. [PubMed]
23 Streptococcus pneumoniae Thanassi JA, et al (2002). Identification of 113 conserved essential genes using a high-throughput gene disruption system in Streptococcus pneumoniae. Nucleic Acids Res, 30:3152-62. [PubMed]

Song JH, et al (2005). Identification of Essential Genes in Streptococcus pneumoniae by Allelic Replacement Mutagenesis. Mol Cells, 19:365-74. [PubMed]
24 Streptococcus sanguinis Xu, P et al(2011). Genome-wide essential gene identification in Streptococcus sanguinis. Sci. Rep. 1, 125; DOI:10.1038/srep00125. [PubMed]
25 Vibrio cholerae N16961 Cameron DE, Urbach MJ,Mekalanos JJ(2008). A defined transposon mutant library and its use in identifying motility genes in Vibrio cholerae. PNAS, 105(25):8736-8741. [PubMed]
No Organism (Eukaryotes) Essential genes Reference
1 Arabidopsis thaliana Meinke D, et al(2008). Identifying essential genes in Arabidopsis thaliana. Trends Plant Sci, 13:483-91. [PubMed]
2 Aspergillus fumigatus Hu W, et al (2007). Essential gene identification and drug target prioritization in Aspergillus fumigatus. PLoS Pathog, 3:e24. [PubMed]
3 Caenorhabditis elegans Kamath RS, et al (2003). Systematic functional analysis of the Caenorhabditis elegans genome using RNAi. Nature, 421:231-7. [PubMed]
4 Danio rerio Amsterdam A, et al (2004). Identification of 315 genes essential for early zebrafish development. Proc Natl Acad Sci U S A, 101:12792-7. [PubMed]
5 Drosophila melanogaster Spradling AC, et al (1999). The Berkeley Drosophila Genome Project gene disruption project: Single P-element insertions mutating 25% of vital Drosophila genes. Genetics, 153:135-77. [PubMed]
6 Homo sapiens Liao BY, Zhang J (2008). Null mutations in human and mouse orthologs frequently result in different phenotypes. Proc Natl Acad Sci U S A, 105:6987-92. [PubMed]
7 Mus musculus Liao BY, Zhang J (2007). Mouse duplicate genes are as essential as singletons. Trends Genet, 23:378-81. [PubMed]
8 Saccharomyces cerevisiae Giaever G, et al (2002). Functional profiling of the Saccharomyces cerevisiae genome. Nature, 418:387-91. [PubMed]
No Organism (Archaea) Essential genes Reference
1 Methanococcus maripaludis S2 Sarmiento F, et al (2013). Genome-scale analysis of gene function in the hydrogenotrophic methanogenic archaeon Methanococcus maripaludis. Proc Natl Acad Sci U S A, 110:4726-31. [PubMed]

 

DEG 7.5 contains 14708 essential genes in the following Organisms ( July 3,2013).
No Organism (Prokaryotes) Essential genes Reference
1 Acinetobacter baylyi ADP1 de Berardinis V, et al (2008). A complete collection of single-gene deletion mutants of Acinetobacter baylyi ADP1. Mol Syst Biol. [PubMed]
2 Bacillus subtilis 168 Kobayashi K, et al (2003). Essential Bacillus subtilis genes. Proc Natl Acad Sci U S A, 100:4678-83. [PubMed]
3 Bacteroides thetaiotaomicron VPI-5482 Goodman Al, et al (2009). Identifying Genetic Determinants Needed to Establish a Human Gut Symbiont in Its Habitat. Cell Host & Microbe, 6: 279¨C289. [PubMed]
4 Burkholderia thailandensis E264 Baugh, Loren, et al. "Combining Functional and Structural Genomics to Sample the Essential Burkholderia Structome." PloS one 8.1 (2013): e53851. [PubMed]
5 Caulobacter crescentus Christen B, et al (2011). The essential genome of a bacterium. Mol Syst Biol, 7:528. [PubMed]
6 Escherichia coli MG1655 Gerdes SY, et al (2003). Experimental determination and system level analysis of essential genes in Escherichia coli MG1655. J Bacteriol, 185:5673-84. [PubMed]

Baba T, et al (2006). Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol. [PubMed]
7 Escherichia coli MG1655 I Gerdes SY, et al (2003). Experimental determination and system level analysis of essential genes in Escherichia coli MG1655. J Bacteriol, 185:5673-84. [PubMed]
8 Escherichia coli MG1655 II Baba T, et al (2006). Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol. [PubMed]
9 Francisella novicida U112 Gallagher LA, et al (2007). A comprehensive transposon mutant library of Francisella novicida, a bioweapon surrogate. Proc Natl Acad Sci U S A, 104:1009-14. [PubMed]
10 Haemophilus influenzae Rd KW20 Akerley BJ, et al (2002). A genome-scale analysis for identification of genes required for growth or survival of Haemophilus influenzae. Proc Natl Acad Sci U S A, 99:966-71. [PubMed]
11 Helicobacter pylori 26695 Salama NR, et al (2004). Global transposon mutagenesis and essential gene analysis of Helicobacter pylori. J Bacteriol, 186:7926-35. [PubMed]
12 Mycobacterium tuberculosis H37Rv Sassetti CM, et al (2003). Genes required for mycobacterial growth defined by high density mutagenesis. Mol Microbiol, 48:77-84. [PubMed]
13 Mycoplasma genitalium G37 Glass JI, et al (2006). Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A, 103:425-30. [PubMed]
14 Mycoplasma pulmonis UAB CTIP French CT, et al (2008). Large-scale transposon mutagenesis of Mycoplasma pulmonis. Mol Microbiol. [PubMed]
15 Porphyromonas gingivalis ATCC 33277 Klein BA, et al (2012). Identification of essential genes of the periodontal pathogen Porphyromonas. BMC Genomics, 13: 578. [PubMed]
16 Pseudomonas aeruginosa UCBPP-PA14 Liberati NT, et al (2006). An ordered, nonredundant library of Pseudomonas aeruginosa strain PA14 transposon insertion mutants. Proc Natl Acad Sci U S A, 103:2833-8. [PubMed]
17 Salmonella enterica serovar Typhi Langridge GC, et al (2009). Simultaneous assay of every Salmonella Typhi gene using one million transposon mutants. Genome Res, 19:2308-16. [PubMed]
18 Salmonella typhimurium LT2 Knuth K, et al (2004). Large-scale identification of essential Salmonella genes by trapping lethal insertions. Mol Microbiol, 51:1729-44. [PubMed]
19 Staphylococcus aureus N315 Ji Y, et al (2001). Identification of critical staphylococcal genes using conditional phenotypes generated by antisense RNA. Science, 293:2266-9. [PubMed]

Forsyth RA, et al (2002). A genome-wide strategy for the identification of essential genes in Staphylococcus aureus. Mol Microbiol, 43:1387-400. [PubMed]

Ko KS, et al (2006). Screening of Essential Genes in Staphylococcus aureus N315 Using Comparative Genomics and Allelic Replacement Mutagenesis. J Microbiol Biotechnol, 16:623-32.
20 Staphylococcus aureus NCTC 8325 Chaudhuri RR, et al (2009). Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH). BMC Genomics, 10:291. [PubMed]
21 Streptococcus pneumoniae Thanassi JA, et al (2002). Identification of 113 conserved essential genes using a high-throughput gene disruption system in Streptococcus pneumoniae. Nucleic Acids Res, 30:3152-62. [PubMed]

Song JH, et al (2005). Identification of Essential Genes in Streptococcus pneumoniae by Allelic Replacement Mutagenesis. Mol Cells, 19:365-74. [PubMed]
22 Streptococcus sanguinis Xu, P et al(2011). Genome-wide essential gene identification in Streptococcus sanguinis. Sci. Rep. 1, 125; DOI:10.1038/srep00125. [PubMed]
23 Vibrio cholerae N16961 Cameron DE, Urbach MJ,Mekalanos JJ(2008). A defined transposon mutant library and its use in identifying motility genes in Vibrio cholerae. PNAS, 105(25):8736-8741. [PubMed]
No Organism (Eukaryotes) Essential genes Reference
1 Arabidopsis thaliana Meinke D, et al(2008). Identifying essential genes in Arabidopsis thaliana. Trends Plant Sci, 13:483-91. [PubMed]
2 Aspergillus fumigatus Hu W, et al (2007). Essential gene identification and drug target prioritization in Aspergillus fumigatus. PLoS Pathog, 3:e24. [PubMed]
3 Caenorhabditis elegans Kamath RS, et al (2003). Systematic functional analysis of the Caenorhabditis elegans genome using RNAi. Nature, 421:231-7. [PubMed]
4 Danio rerio Amsterdam A, et al (2004). Identification of 315 genes essential for early zebrafish development. Proc Natl Acad Sci U S A, 101:12792-7. [PubMed]
5 Drosophila melanogaster Spradling AC, et al (1999). The Berkeley Drosophila Genome Project gene disruption project: Single P-element insertions mutating 25% of vital Drosophila genes. Genetics, 153:135-77. [PubMed]
6 Homo sapiens Liao BY, Zhang J (2008). Null mutations in human and mouse orthologs frequently result in different phenotypes. Proc Natl Acad Sci U S A, 105:6987-92. [PubMed]
7 Mus musculus Liao BY, Zhang J (2007). Mouse duplicate genes are as essential as singletons. Trends Genet, 23:378-81. [PubMed]
8 Saccharomyces cerevisiae Giaever G, et al (2002). Functional profiling of the Saccharomyces cerevisiae genome. Nature, 418:387-91. [PubMed]
No Organism (Archaea) Essential genes Reference
1 Methanococcus maripaludis S2 Sarmiento F, et al (2013). Genome-scale analysis of gene function in the hydrogenotrophic methanogenic archaeon Methanococcus maripaludis. Proc Natl Acad Sci U S A, 110:4726-31. [PubMed]

 

DEG 7.0 contains 13783 essential genes in the following Organisms ( November 11,2012).
No Organism (Prokaryotes) Essential genes Reference
1 Acinetobacter baylyi ADP1 de Berardinis V, et al (2008). A complete collection of single-gene deletion mutants of Acinetobacter baylyi ADP1. Mol Syst Biol. [PubMed]
2 Bacillus subtilis 168 Kobayashi K, et al (2003). Essential Bacillus subtilis genes. Proc Natl Acad Sci U S A, 100:4678-83. [PubMed]
3 Bacteroides thetaiotaomicron VPI-5482 Goodman Al, et al (2009). Identifying Genetic Determinants Needed to Establish a Human Gut Symbiont in Its Habitat. Cell Host & Microbe, 6: 279¨C289. [PubMed]
4 Burkholderia thailandensis E264 Baugh, Loren, et al. "Combining Functional and Structural Genomics to Sample the Essential Burkholderia Structome." PloS one 8.1 (2013): e53851. [PubMed]
5 Caulobacter crescentus Christen B, et al (2011). The essential genome of a bacterium. Mol Syst Biol, 7:528. [PubMed]
6 Escherichia coli MG1655 Gerdes SY, et al (2003). Experimental determination and system level analysis of essential genes in Escherichia coli MG1655. J Bacteriol, 185:5673-84. [PubMed]

Baba T, et al (2006). Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol. [PubMed]
7 Escherichia coli MG1655 I Gerdes SY, et al (2003). Experimental determination and system level analysis of essential genes in Escherichia coli MG1655. J Bacteriol, 185:5673-84. [PubMed]
8 Escherichia coli MG1655 II Baba T, et al (2006). Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol. [PubMed]
9 Francisella novicida U112 Gallagher LA, et al (2007). A comprehensive transposon mutant library of Francisella novicida, a bioweapon surrogate. Proc Natl Acad Sci U S A, 104:1009-14. [PubMed]
10 Haemophilus influenzae Rd KW20 Akerley BJ, et al (2002). A genome-scale analysis for identification of genes required for growth or survival of Haemophilus influenzae. Proc Natl Acad Sci U S A, 99:966-71. [PubMed]
11 Helicobacter pylori 26695 Salama NR, et al (2004). Global transposon mutagenesis and essential gene analysis of Helicobacter pylori. J Bacteriol, 186:7926-35. [PubMed]
12 Mycobacterium tuberculosis H37Rv Sassetti CM, et al (2003). Genes required for mycobacterial growth defined by high density mutagenesis. Mol Microbiol, 48:77-84. [PubMed]
13 Mycoplasma genitalium G37 Glass JI, et al (2006). Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A, 103:425-30. [PubMed]
14 Mycoplasma pulmonis UAB CTIP French CT, et al (2008). Large-scale transposon mutagenesis of Mycoplasma pulmonis. Mol Microbiol. [PubMed]
15 Porphyromonas gingivalis ATCC 33277 Klein BA, et al (2012). Identification of essential genes of the periodontal pathogen Porphyromonas. BMC Genomics, 13: 578. [PubMed]
16 Pseudomonas aeruginosa UCBPP-PA14 Liberati NT, et al (2006). An ordered, nonredundant library of Pseudomonas aeruginosa strain PA14 transposon insertion mutants. Proc Natl Acad Sci U S A, 103:2833-8. [PubMed]
17 Salmonella enterica serovar Typhi Langridge GC, et al (2009). Simultaneous assay of every Salmonella Typhi gene using one million transposon mutants. Genome Res, 19:2308-16. [PubMed]
18 Salmonella typhimurium LT2 Knuth K, et al (2004). Large-scale identification of essential Salmonella genes by trapping lethal insertions. Mol Microbiol, 51:1729-44. [PubMed]
19 Staphylococcus aureus N315 Ji Y, et al (2001). Identification of critical staphylococcal genes using conditional phenotypes generated by antisense RNA. Science, 293:2266-9. [PubMed]

Forsyth RA, et al (2002). A genome-wide strategy for the identification of essential genes in Staphylococcus aureus. Mol Microbiol, 43:1387-400. [PubMed]

Ko KS, et al (2006). Screening of Essential Genes in Staphylococcus aureus N315 Using Comparative Genomics and Allelic Replacement Mutagenesis. J Microbiol Biotechnol, 16:623-32.
20 Staphylococcus aureus NCTC 8325 Chaudhuri RR, et al (2009). Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH). BMC Genomics, 10:291. [PubMed]
21 Streptococcus pneumoniae Thanassi JA, et al (2002). Identification of 113 conserved essential genes using a high-throughput gene disruption system in Streptococcus pneumoniae. Nucleic Acids Res, 30:3152-62. [PubMed]

Song JH, et al (2005). Identification of Essential Genes in Streptococcus pneumoniae by Allelic Replacement Mutagenesis. Mol Cells, 19:365-74. [PubMed]
22 Streptococcus sanguinis Xu, P et al(2011). Genome-wide essential gene identification in Streptococcus sanguinis. Sci. Rep. 1, 125; DOI:10.1038/srep00125. [PubMed]
23 Vibrio cholerae N16961 Cameron DE, Urbach MJ,Mekalanos JJ(2008). A defined transposon mutant library and its use in identifying motility genes in Vibrio cholerae. PNAS, 105(25):8736-8741. [PubMed]
No Organism (Eukaryotes) Essential genes Reference
1 Arabidopsis thaliana Meinke D, et al(2008). Identifying essential genes in Arabidopsis thaliana. Trends Plant Sci, 13:483-91. [PubMed]
2 Aspergillus fumigatus Hu W, et al (2007). Essential gene identification and drug target prioritization in Aspergillus fumigatus. PLoS Pathog, 3:e24. [PubMed]
3 Caenorhabditis elegans Kamath RS, et al (2003). Systematic functional analysis of the Caenorhabditis elegans genome using RNAi. Nature, 421:231-7. [PubMed]
4 Danio rerio Amsterdam A, et al (2004). Identification of 315 genes essential for early zebrafish development. Proc Natl Acad Sci U S A, 101:12792-7. [PubMed]
5 Drosophila melanogaster Spradling AC, et al (1999). The Berkeley Drosophila Genome Project gene disruption project: Single P-element insertions mutating 25% of vital Drosophila genes. Genetics, 153:135-77. [PubMed]
6 Homo sapiens Liao BY, Zhang J (2008). Null mutations in human and mouse orthologs frequently result in different phenotypes. Proc Natl Acad Sci U S A, 105:6987-92. [PubMed]
7 Mus musculus Liao BY, Zhang J (2007). Mouse duplicate genes are as essential as singletons. Trends Genet, 23:378-81. [PubMed]
8 Saccharomyces cerevisiae Giaever G, et al (2002). Functional profiling of the Saccharomyces cerevisiae genome. Nature, 418:387-91. [PubMed]

 

DEG 6.8 contains 12995 essential genes in the following Organisms ( November 4,2011).
No Organism (Prokaryotes) Essential genes Reference
1 Acinetobacter baylyi ADP1 de Berardinis V, et al (2008). A complete collection of single-gene deletion mutants of Acinetobacter baylyi ADP1. Mol Syst Biol. [PubMed]
2 Bacillus subtilis 168 Kobayashi K, et al (2003). Essential Bacillus subtilis genes. Proc Natl Acad Sci U S A, 100:4678-83. [PubMed]
3 Caulobacter crescentus Christen B, et al (2011). The essential genome of a bacterium. Mol Syst Biol, 7:528. [PubMed]
4 Escherichia coli MG1655 Gerdes SY, et al (2003). Experimental determination and system level analysis of essential genes in Escherichia coli MG1655. J Bacteriol, 185:5673-84. [PubMed]

Baba T, et al (2006). Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol. [PubMed]
5 Escherichia coli MG1655 I Gerdes SY, et al (2003). Experimental determination and system level analysis of essential genes in Escherichia coli MG1655. J Bacteriol, 185:5673-84. [PubMed]
6 Escherichia coli MG1655 II Baba T, et al (2006). Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol. [PubMed]
7 Francisella novicida U112 Gallagher LA, et al (2007). A comprehensive transposon mutant library of Francisella novicida, a bioweapon surrogate. Proc Natl Acad Sci U S A, 104:1009-14. [PubMed]
8 Haemophilus influenzae Rd KW20 Akerley BJ, et al (2002). A genome-scale analysis for identification of genes required for growth or survival of Haemophilus influenzae. Proc Natl Acad Sci U S A, 99:966-71. [PubMed]
9 Helicobacter pylori 26695 Salama NR, et al (2004). Global transposon mutagenesis and essential gene analysis of Helicobacter pylori. J Bacteriol, 186:7926-35. [PubMed]
10 Mycobacterium tuberculosis H37Rv Sassetti CM, et al (2003). Genes required for mycobacterial growth defined by high density mutagenesis. Mol Microbiol, 48:77-84. [PubMed]
11 Mycoplasma genitalium G37 Glass JI, et al (2006). Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A, 103:425-30. [PubMed]
12 Mycoplasma pulmonis UAB CTIP French CT, et al (2008). Large-scale transposon mutagenesis of Mycoplasma pulmonis. Mol Microbiol. [PubMed]
13 Pseudomonas aeruginosa UCBPP-PA14 Liberati NT, et al (2006). An ordered, nonredundant library of Pseudomonas aeruginosa strain PA14 transposon insertion mutants. Proc Natl Acad Sci U S A, 103:2833-8. [PubMed]
14 Salmonella enterica serovar Typhi Langridge GC, et al (2009). Simultaneous assay of every Salmonella Typhi gene using one million transposon mutants. Genome Res, 19:2308-16. [PubMed]
15 Salmonella typhimurium LT2 Knuth K, et al (2004). Large-scale identification of essential Salmonella genes by trapping lethal insertions. Mol Microbiol, 51:1729-44. [PubMed]
16 Staphylococcus aureus N315 Ji Y, et al (2001). Identification of critical staphylococcal genes using conditional phenotypes generated by antisense RNA. Science, 293:2266-9. [PubMed]

Forsyth RA, et al (2002). A genome-wide strategy for the identification of essential genes in Staphylococcus aureus. Mol Microbiol, 43:1387-400. [PubMed]

Ko KS, et al (2006). Screening of Essential Genes in Staphylococcus aureus N315 Using Comparative Genomics and Allelic Replacement Mutagenesis. J Microbiol Biotechnol, 16:623-32.
17 Staphylococcus aureus NCTC 8325 Chaudhuri RR, et al (2009). Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH). BMC Genomics, 10:291. [PubMed]
18 Streptococcus pneumoniae Thanassi JA, et al (2002). Identification of 113 conserved essential genes using a high-throughput gene disruption system in Streptococcus pneumoniae. Nucleic Acids Res, 30:3152-62. [PubMed]

Song JH, et al (2005). Identification of Essential Genes in Streptococcus pneumoniae by Allelic Replacement Mutagenesis. Mol Cells, 19:365-74. [PubMed]
19 Streptococcus sanguinis Xu, P et al(2011). Genome-wide essential gene identification in Streptococcus sanguinis. Sci. Rep. 1, 125; DOI:10.1038/srep00125. [PubMed]
20 Vibrio cholerae N16961 Cameron DE, Urbach MJ,Mekalanos JJ(2008). A defined transposon mutant library and its use in identifying motility genes in Vibrio cholerae. PNAS, 105(25):8736-8741. [PubMed]
No Organism (Eukaryotes) Essential genes Reference
1 Arabidopsis thaliana Meinke D, et al(2008). Identifying essential genes in Arabidopsis thaliana. Trends Plant Sci, 13:483-91. [PubMed]
2 Aspergillus fumigatus Hu W, et al (2007). Essential gene identification and drug target prioritization in Aspergillus fumigatus. PLoS Pathog, 3:e24. [PubMed]
3 Caenorhabditis elegans Kamath RS, et al (2003). Systematic functional analysis of the Caenorhabditis elegans genome using RNAi. Nature, 421:231-7. [PubMed]
4 Danio rerio Amsterdam A, et al (2004). Identification of 315 genes essential for early zebrafish development. Proc Natl Acad Sci U S A, 101:12792-7. [PubMed]
5 Drosophila melanogaster Spradling AC, et al (1999). The Berkeley Drosophila Genome Project gene disruption project: Single P-element insertions mutating 25% of vital Drosophila genes. Genetics, 153:135-77. [PubMed]
6 Homo sapiens Liao BY, Zhang J (2008). Null mutations in human and mouse orthologs frequently result in different phenotypes. Proc Natl Acad Sci U S A, 105:6987-92. [PubMed]
7 Mus musculus Liao BY, Zhang J (2007). Mouse duplicate genes are as essential as singletons. Trends Genet, 23:378-81. [PubMed]
8 Saccharomyces cerevisiae Giaever G, et al (2002). Functional profiling of the Saccharomyces cerevisiae genome. Nature, 418:387-91. [PubMed]

 

DEG 6.7 contains 12777 essential genes in the following Organisms (October 9,2011).
No Organism (Prokaryotes) Essential genes Reference
1 Acinetobacter baylyi ADP1 de Berardinis V, et al (2008). A complete collection of single-gene deletion mutants of Acinetobacter baylyi ADP1. Mol Syst Biol. [PubMed]
2 Bacillus subtilis 168 Kobayashi K, et al (2003). Essential Bacillus subtilis genes. Proc Natl Acad Sci U S A, 100:4678-83. [PubMed]
3 Caulobacter crescentus Christen B, et al (2011). The essential genome of a bacterium. Mol Syst Biol, 7:528. [PubMed]
4 Escherichia coli MG1655 Gerdes SY, et al (2003). Experimental determination and system level analysis of essential genes in Escherichia coli MG1655. J Bacteriol, 185:5673-84. [PubMed]

Baba T, et al (2006). Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol. [PubMed]
5 Escherichia coli MG1655 I Gerdes SY, et al (2003). Experimental determination and system level analysis of essential genes in Escherichia coli MG1655. J Bacteriol, 185:5673-84. [PubMed]
6 Escherichia coli MG1655 II Baba T, et al (2006). Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol. [PubMed]
7 Francisella novicida U112 Gallagher LA, et al (2007). A comprehensive transposon mutant library of Francisella novicida, a bioweapon surrogate. Proc Natl Acad Sci U S A, 104:1009-14. [PubMed]
8 Haemophilus influenzae Rd KW20 Akerley BJ, et al (2002). A genome-scale analysis for identification of genes required for growth or survival of Haemophilus influenzae. Proc Natl Acad Sci U S A, 99:966-71. [PubMed]
9 Helicobacter pylori 26695 Salama NR, et al (2004). Global transposon mutagenesis and essential gene analysis of Helicobacter pylori. J Bacteriol, 186:7926-35. [PubMed]
10 Mycobacterium tuberculosis H37Rv Sassetti CM, et al (2003). Genes required for mycobacterial growth defined by high density mutagenesis. Mol Microbiol, 48:77-84. [PubMed]
11 Mycoplasma genitalium G37 Glass JI, et al (2006). Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A, 103:425-30. [PubMed]
12 Mycoplasma pulmonis UAB CTIP French CT, et al (2008). Large-scale transposon mutagenesis of Mycoplasma pulmonis. Mol Microbiol. [PubMed]
13 Pseudomonas aeruginosa UCBPP-PA14 Liberati NT, et al (2006). An ordered, nonredundant library of Pseudomonas aeruginosa strain PA14 transposon insertion mutants. Proc Natl Acad Sci U S A, 103:2833-8. [PubMed]
14 Salmonella enterica serovar Typhi Langridge GC, et al (2009). Simultaneous assay of every Salmonella Typhi gene using one million transposon mutants. Genome Res, 19:2308-16. [PubMed]
15 Salmonella typhimurium LT2 Knuth K, et al (2004). Large-scale identification of essential Salmonella genes by trapping lethal insertions. Mol Microbiol, 51:1729-44. [PubMed]
16 Staphylococcus aureus N315 Ji Y, et al (2001). Identification of critical staphylococcal genes using conditional phenotypes generated by antisense RNA. Science, 293:2266-9. [PubMed]

Forsyth RA, et al (2002). A genome-wide strategy for the identification of essential genes in Staphylococcus aureus. Mol Microbiol, 43:1387-400. [PubMed]

Ko KS, et al (2006). Screening of Essential Genes in Staphylococcus aureus N315 Using Comparative Genomics and Allelic Replacement Mutagenesis. J Microbiol Biotechnol, 16:623-32.
17 Staphylococcus aureus NCTC 8325 Chaudhuri RR, et al (2009). Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH). BMC Genomics, 10:291. [PubMed]
18 Streptococcus pneumoniae Thanassi JA, et al (2002). Identification of 113 conserved essential genes using a high-throughput gene disruption system in Streptococcus pneumoniae. Nucleic Acids Res, 30:3152-62. [PubMed]

Song JH, et al (2005). Identification of Essential Genes in Streptococcus pneumoniae by Allelic Replacement Mutagenesis. Mol Cells, 19:365-74. [PubMed]
19 Vibrio cholerae N16961 Cameron DE, Urbach MJ,Mekalanos JJ(2008). A defined transposon mutant library and its use in identifying motility genes in Vibrio cholerae. PNAS, 105(25):8736-8741. [PubMed]
No Organism (Eukaryotes) Essential genes Reference
1 Arabidopsis thaliana Meinke D, et al(2008). Identifying essential genes in Arabidopsis thaliana. Trends Plant Sci, 13:483-91. [PubMed]
2 Aspergillus fumigatus Hu W, et al (2007). Essential gene identification and drug target prioritization in Aspergillus fumigatus. PLoS Pathog, 3:e24. [PubMed]
3 Caenorhabditis elegans Kamath RS, et al (2003). Systematic functional analysis of the Caenorhabditis elegans genome using RNAi. Nature, 421:231-7. [PubMed]
4 Danio rerio Amsterdam A, et al (2004). Identification of 315 genes essential for early zebrafish development. Proc Natl Acad Sci U S A, 101:12792-7. [PubMed]
5 Drosophila melanogaster Spradling AC, et al (1999). The Berkeley Drosophila Genome Project gene disruption project: Single P-element insertions mutating 25% of vital Drosophila genes. Genetics, 153:135-77. [PubMed]
6 Homo sapiens Liao BY, Zhang J (2008). Null mutations in human and mouse orthologs frequently result in different phenotypes. Proc Natl Acad Sci U S A, 105:6987-92. [PubMed]
7 Mus musculus Liao BY, Zhang J (2007). Mouse duplicate genes are as essential as singletons. Trends Genet, 23:378-81. [PubMed]
8 Saccharomyces cerevisiae Giaever G, et al (2002). Functional profiling of the Saccharomyces cerevisiae genome. Nature, 418:387-91. [PubMed]


DEG 6.5 contains 12297 essential genes in the following Organisms (March 29,2011).
No Organism (Prokaryotes) Essential genes Reference
1 Acinetobacter baylyi ADP1 de Berardinis V, et al (2008). A complete collection of single-gene deletion mutants of Acinetobacter baylyi ADP1. Mol Syst Biol. [PubMed]
2 Bacillus subtilis 168 Kobayashi K, et al (2003). Essential Bacillus subtilis genes. Proc Natl Acad Sci U S A, 100:4678-83. [PubMed]
3 Escherichia coli MG1655 Gerdes SY, et al (2003). Experimental determination and system level analysis of essential genes in Escherichia coli MG1655. J Bacteriol, 185:5673-84. [PubMed]

Baba T, et al (2006). Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol. [PubMed]
4 Escherichia coli MG1655 I Gerdes SY, et al (2003). Experimental determination and system level analysis of essential genes in Escherichia coli MG1655. J Bacteriol, 185:5673-84. [PubMed]
5 Escherichia coli MG1655 II Baba T, et al (2006). Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol. [PubMed]
6 Francisella novicida U112 Gallagher LA, et al (2007). A comprehensive transposon mutant library of Francisella novicida, a bioweapon surrogate. Proc Natl Acad Sci U S A, 104:1009-14. [PubMed]
7 Haemophilus influenzae Rd KW20 Akerley BJ, et al (2002). A genome-scale analysis for identification of genes required for growth or survival of Haemophilus influenzae. Proc Natl Acad Sci U S A, 99:966-71. [PubMed]
8 Helicobacter pylori 26695 Salama NR, et al (2004). Global transposon mutagenesis and essential gene analysis of Helicobacter pylori. J Bacteriol, 186:7926-35. [PubMed]
9 Mycobacterium tuberculosis H37Rv Sassetti CM, et al (2003). Genes required for mycobacterial growth defined by high density mutagenesis. Mol Microbiol, 48:77-84. [PubMed]
10 Mycoplasma genitalium G37 Glass JI, et al (2006). Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A, 103:425-30. [PubMed]
11 Mycoplasma pulmonis UAB CTIP French CT, et al (2008). Large-scale transposon mutagenesis of Mycoplasma pulmonis. Mol Microbiol. [PubMed]
12 Pseudomonas aeruginosa UCBPP-PA14 Liberati NT, et al (2006). An ordered, nonredundant library of Pseudomonas aeruginosa strain PA14 transposon insertion mutants. Proc Natl Acad Sci U S A, 103:2833-8. [PubMed]
13 Salmonella enterica serovar Typhi Langridge GC, et al (2009). Simultaneous assay of every Salmonella Typhi gene using one million transposon mutants. Genome Res, 19:2308-16. [PubMed]
14 Salmonella typhimurium LT2 Knuth K, et al (2004). Large-scale identification of essential Salmonella genes by trapping lethal insertions. Mol Microbiol, 51:1729-44. [PubMed]
15 Staphylococcus aureus N315 Ji Y, et al (2001). Identification of critical staphylococcal genes using conditional phenotypes generated by antisense RNA. Science, 293:2266-9. [PubMed]

Forsyth RA, et al (2002). A genome-wide strategy for the identification of essential genes in Staphylococcus aureus. Mol Microbiol, 43:1387-400. [PubMed]

Ko KS, et al (2006). Screening of Essential Genes in Staphylococcus aureus N315 Using Comparative Genomics and Allelic Replacement Mutagenesis. J Microbiol Biotechnol, 16:623-32.
16 Staphylococcus aureus NCTC 8325 Chaudhuri RR, et al (2009). Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH). BMC Genomics, 10:291. [PubMed]
17 Streptococcus pneumoniae Thanassi JA, et al (2002). Identification of 113 conserved essential genes using a high-throughput gene disruption system in Streptococcus pneumoniae. Nucleic Acids Res, 30:3152-62. [PubMed]

Song JH, et al (2005). Identification of Essential Genes in Streptococcus pneumoniae by Allelic Replacement Mutagenesis. Mol Cells, 19:365-74. [PubMed]
18 Vibrio cholerae N16961 Cameron DE, Urbach MJ,Mekalanos JJ(2008). A defined transposon mutant library and its use in identifying motility genes in Vibrio cholerae. PNAS, 105(25):8736-8741. [PubMed]
No Organism (Eukaryotes) Essential genes Reference
1 Arabidopsis thaliana Meinke D, et al(2008). Identifying essential genes in Arabidopsis thaliana. Trends Plant Sci, 13:483-91. [PubMed]
2 Aspergillus fumigatus Hu W, et al (2007). Essential gene identification and drug target prioritization in Aspergillus fumigatus. PLoS Pathog, 3:e24. [PubMed]
3 Caenorhabditis elegans Kamath RS, et al (2003). Systematic functional analysis of the Caenorhabditis elegans genome using RNAi. Nature, 421:231-7. [PubMed]
4 Danio rerio Amsterdam A, et al (2004). Identification of 315 genes essential for early zebrafish development. Proc Natl Acad Sci U S A, 101:12792-7. [PubMed]
5 Drosophila melanogaster Spradling AC, et al (1999). The Berkeley Drosophila Genome Project gene disruption project: Single P-element insertions mutating 25% of vital Drosophila genes. Genetics, 153:135-77. [PubMed]
6 Homo sapiens Liao BY, Zhang J (2008). Null mutations in human and mouse orthologs frequently result in different phenotypes. Proc Natl Acad Sci U S A, 105:6987-92. [PubMed]
7 Mus musculus Liao BY, Zhang J (2007). Mouse duplicate genes are as essential as singletons. Trends Genet, 23:378-81. [PubMed]
8 Saccharomyces cerevisiae Giaever G, et al (2002). Functional profiling of the Saccharomyces cerevisiae genome. Nature, 418:387-91. [PubMed]

 

DEG 6.1 contains 10618 essential genes in the following Organisms (November 4,2010).
No Organism (Prokaryotes) Essential genes Reference
1 Acinetobacter baylyi ADP1
499
de Berardinis V, et al (2008). A complete collection of single-gene deletion mutants of Acinetobacter baylyi ADP1. Mol Syst Biol. [PubMed]
2 Bacillus subtilis 168
271
Kobayashi K, et al (2003). Essential Bacillus subtilis genes. Proc Natl Acad Sci U S A, 100:4678-83. [PubMed]
3 Escherichia coli MG1655
712
Gerdes SY, et al (2003). Experimental determination and system level analysis of essential genes in Escherichia coli MG1655. J Bacteriol, 185:5673-84. [PubMed]

Baba T, et al (2006). Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol. [PubMed]
4 Francisella novicida U112
392
Gallagher LA, et al (2007). A comprehensive transposon mutant library of Francisella novicida, a bioweapon surrogate. Proc Natl Acad Sci U S A, 104:1009-14. [PubMed]
5 Haemophilus influenzae Rd KW20
642
Akerley BJ, et al (2002). A genome-scale analysis for identification of genes required for growth or survival of Haemophilus influenzae. Proc Natl Acad Sci U S A, 99:966-71. [PubMed]
6 Helicobacter pylori 26695
323
Salama NR, et al (2004). Global transposon mutagenesis and essential gene analysis of Helicobacter pylori. J Bacteriol, 186:7926-35. [PubMed]
7 Mycobacterium tuberculosis H37Rv
614
Sassetti CM, et al (2003). Genes required for mycobacterial growth defined by high density mutagenesis. Mol Microbiol, 48:77-84. [PubMed]
8 Mycoplasma genitalium G37
381
Glass JI, et al (2006). Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A, 103:425-30. [PubMed]
9 Mycoplasma pulmonis UAB CTIP
310
French CT, et al (2008). Large-scale transposon mutagenesis of Mycoplasma pulmonis. Mol Microbiol. [PubMed]
10 Pseudomonas aeruginosa UCBPP-PA14
335
Liberati NT, et al (2006). An ordered, nonredundant library of Pseudomonas aeruginosa strain PA14 transposon insertion mutants. Proc Natl Acad Sci U S A, 103:2833-8. [PubMed]
11 Salmonella enterica serovar Typhi
353
Langridge GC, et al (2009). Simultaneous assay of every Salmonella Typhi gene using one million transposon mutants. Genome Res, 19:2308-16. [PubMed]
12 Salmonella typhimurium LT2
230
Knuth K, et al (2004). Large-scale identification of essential Salmonella genes by trapping lethal insertions. Mol Microbiol, 51:1729-44. [PubMed]
13 Staphylococcus aureus N315
302
Ji Y, et al (2001). Identification of critical staphylococcal genes using conditional phenotypes generated by antisense RNA. Science, 293:2266-9. [PubMed]

Forsyth RA, et al (2002). A genome-wide strategy for the identification of essential genes in Staphylococcus aureus. Mol Microbiol, 43:1387-400. [PubMed]

Ko KS, et al (2006). Screening of Essential Genes in Staphylococcus aureus N315 Using Comparative Genomics and Allelic Replacement Mutagenesis. J Microbiol Biotechnol, 16:623-32.
14 Staphylococcus aureus NCTC 8325
351
Chaudhuri RR, et al (2009). Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH). BMC Genomics, 10:291. [PubMed]
15 Streptococcus pneumoniae
244
Thanassi JA, et al (2002). Identification of 113 conserved essential genes using a high-throughput gene disruption system in Streptococcus pneumoniae. Nucleic Acids Res, 30:3152-62. [PubMed]

Song JH, et al (2005). Identification of Essential Genes in Streptococcus pneumoniae by Allelic Replacement Mutagenesis. Mol Cells, 19:365-74. [PubMed]
16 Vibrio cholerae
5
Judson N, Mekalanos JJ (2000). TnAraOut, a transposon-based approach to identify and characterize essential bacterial genes. Nat Biotechnol, 18:740-5. [PubMed]
No Organism (Eukaryotes) Essential genes Reference
1 Arabidopsis thaliana
356
Meinke D, et al(2008). Identifying essential genes in Arabidopsis thaliana. Trends Plant Sci, 13:483-91. [PubMed]
2 Aspergillus fumigatus
35
Hu W, et al (2007). Essential gene identification and drug target prioritization in Aspergillus fumigatus. PLoS Pathog, 3:e24. [PubMed]
3 Caenorhabditis elegans
294
Kamath RS, et al (2003). Systematic functional analysis of the Caenorhabditis elegans genome using RNAi. Nature, 421:231-7. [PubMed]
4 Danio rerio
288
Amsterdam A, et al (2004). Identification of 315 genes essential for early zebrafish development. Proc Natl Acad Sci U S A, 101:12792-7. [PubMed]
5 Drosophila melanogaster
339
Spradling AC, et al (1999). The Berkeley Drosophila Genome Project gene disruption project: Single P-element insertions mutating 25% of vital Drosophila genes. Genetics, 153:135-77. [PubMed]
6 Homo sapiens
118
Liao BY, Zhang J (2008). Null mutations in human and mouse orthologs frequently result in different phenotypes. Proc Natl Acad Sci U S A, 105:6987-92. [PubMed]
7 Mus musculus
2114
Liao BY, Zhang J (2007). Mouse duplicate genes are as essential as singletons. Trends Genet, 23:378-81. [PubMed]
8 Saccharomyces cerevisiae
1110
Giaever G, et al (2002). Functional profiling of the Saccharomyces cerevisiae genome. Nature, 418:387-91. [PubMed]

 

DEG 6.0 contains 11039 essential genes in the following Organisms (October 17, 2010).
No Organism (Prokaryotes) Essential genes Reference
1 Acinetobacter baylyi ADP1
499
de Berardinis V, et al (2008). A complete collection of single-gene deletion mutants of Acinetobacter baylyi ADP1. Mol Syst Biol. [PubMed]
2 Bacillus subtilis 168
271
Kobayashi K, et al (2003). Essential Bacillus subtilis genes. Proc Natl Acad Sci U S A, 100:4678-83. [PubMed]
3 Escherichia coli MG1655
712
Gerdes SY, et al (2003). Experimental determination and system level analysis of essential genes in Escherichia coli MG1655. J Bacteriol, 185:5673-84. [PubMed]

Baba T, et al (2006). Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol. [PubMed]
4 Francisella novicida U112
392
Gallagher LA, et al (2007). A comprehensive transposon mutant library of Francisella novicida, a bioweapon surrogate. Proc Natl Acad Sci U S A, 104:1009-14. [PubMed]
5 Haemophilus influenzae Rd KW20
642
Akerley BJ, et al (2002). A genome-scale analysis for identification of genes required for growth or survival of Haemophilus influenzae. Proc Natl Acad Sci U S A, 99:966-71. [PubMed]
6 Helicobacter pylori 26695
323
Salama NR, et al (2004). Global transposon mutagenesis and essential gene analysis of Helicobacter pylori. J Bacteriol, 186:7926-35. [PubMed]
7 Mycobacterium tuberculosis H37Rv
614
Sassetti CM, et al (2003). Genes required for mycobacterial growth defined by high density mutagenesis. Mol Microbiol, 48:77-84. [PubMed]
8 Mycoplasma genitalium G37
381
Glass JI, et al (2006). Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A, 103:425-30. [PubMed]
9 Mycoplasma pulmonis UAB CTIP
310
French CT, et al (2008). Large-scale transposon mutagenesis of Mycoplasma pulmonis. Mol Microbiol. [PubMed]
10 Pseudomonas aeruginosa UCBPP-PA14
335
Liberati NT, et al (2006). An ordered, nonredundant library of Pseudomonas aeruginosa strain PA14 transposon insertion mutants. Proc Natl Acad Sci U S A, 103:2833-8. [PubMed]
11 Salmonella enterica serovar Typhi
353
Langridge GC, et al (2009). Simultaneous assay of every Salmonella Typhi gene using one million transposon mutants. Genome Res, 19:2308-16. [PubMed]
12 Salmonella typhimurium LT2
230
Knuth K, et al (2004). Large-scale identification of essential Salmonella genes by trapping lethal insertions. Mol Microbiol, 51:1729-44. [PubMed]
13 Staphylococcus aureus N315
302
Ji Y, et al (2001). Identification of critical staphylococcal genes using conditional phenotypes generated by antisense RNA. Science, 293:2266-9. [PubMed]

Forsyth RA, et al (2002). A genome-wide strategy for the identification of essential genes in Staphylococcus aureus. Mol Microbiol, 43:1387-400. [PubMed]

Ko KS, et al (2006). Screening of Essential Genes in Staphylococcus aureus N315 Using Comparative Genomics and Allelic Replacement Mutagenesis. J Microbiol Biotechnol, 16:623-32.
14 Staphylococcus aureus NCTC 8325
351
Chaudhuri RR, et al (2009). Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH). BMC Genomics, 10:291. [PubMed]
15 Streptococcus pneumoniae
244
Thanassi JA, et al (2002). Identification of 113 conserved essential genes using a high-throughput gene disruption system in Streptococcus pneumoniae. Nucleic Acids Res, 30:3152-62. [PubMed]

Song JH, et al (2005). Identification of Essential Genes in Streptococcus pneumoniae by Allelic Replacement Mutagenesis. Mol Cells, 19:365-74. [PubMed]
16 Vibrio cholerae
5
Judson N, Mekalanos JJ (2000). TnAraOut, a transposon-based approach to identify and characterize essential bacterial genes. Nat Biotechnol, 18:740-5. [PubMed]
No Organism (Eukaryotes) Essential genes Reference
1 Arabidopsis thaliana
777
Tzafrir I, et al (2004). Identification of genes required for embryo development in Arabidopsis. Plant Physiol, 135:1206-20.[PubMed]
2 Aspergillus fumigatus
35
Hu W, et al (2007). Essential gene identification and drug target prioritization in Aspergillus fumigatus. PLoS Pathog, 3:e24. [PubMed]
3 Caenorhabditis elegans
294
Kamath RS, et al (2003). Systematic functional analysis of the Caenorhabditis elegans genome using RNAi. Nature, 421:231-7. [PubMed]
4 Danio rerio
288
Amsterdam A, et al (2004). Identification of 315 genes essential for early zebrafish development. Proc Natl Acad Sci U S A, 101:12792-7. [PubMed]
5 Drosophila melanogaster
339
Spradling AC, et al (1999). The Berkeley Drosophila Genome Project gene disruption project: Single P-element insertions mutating 25% of vital Drosophila genes. Genetics, 153:135-77. [PubMed]
6 Homo sapiens
118
Liao BY, Zhang J (2008). Null mutations in human and mouse orthologs frequently result in different phenotypes. Proc Natl Acad Sci U S A, 105:6987-92. [PubMed]
7 Mus musculus
2114
Liao BY, Zhang J (2007). Mouse duplicate genes are as essential as singletons. Trends Genet, 23:378-81. [PubMed]
8 Saccharomyces cerevisiae
1110
Giaever G, et al (2002). Functional profiling of the Saccharomyces cerevisiae genome. Nature, 418:387-91. [PubMed]

 

DEG 5.4 contains 10300 essential genes in the following Organisms (May 28, 2009).
No Organism (Prokaryotes) Essential genes Reference
1 Acinetobacter baylyi ADP1
499
de Berardinis V, et al (2008). A complete collection of single-gene deletion mutants of Acinetobacter baylyi ADP1. Mol Syst Biol. [PubMed]
2 Bacillus subtilis 168
271
Kobayashi K, et al (2003). Essential Bacillus subtilis genes. Proc Natl Acad Sci U S A, 100:4678-83. [PubMed]
3 Escherichia coli MG1655
712
Gerdes SY, et al (2003). Experimental determination and system level analysis of essential genes in Escherichia coli MG1655. J Bacteriol, 185:5673-84. [PubMed]

Baba T, et al (2006). Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol. [PubMed]
4 Francisella novicida U112
392
Gallagher LA, et al (2007). A comprehensive transposon mutant library of Francisella novicida, a bioweapon surrogate. Proc Natl Acad Sci U S A, 104:1009-14. [PubMed]
5 Haemophilus influenzae Rd KW20
642
Akerley BJ, et al (2002). A genome-scale analysis for identification of genes required for growth or survival of Haemophilus influenzae. Proc Natl Acad Sci U S A, 99:966-71. [PubMed]
6 Helicobacter pylori 26695
323
Salama NR, et al (2004). Global transposon mutagenesis and essential gene analysis of Helicobacter pylori. J Bacteriol, 186:7926-35. [PubMed]
7 Mycobacterium tuberculosis H37Rv
614
Sassetti CM, et al (2003). Genes required for mycobacterial growth defined by high density mutagenesis. Mol Microbiol, 48:77-84. [PubMed]
8 Mycoplasma genitalium G37
381
Glass JI, et al (2006). Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A, 103:425-30. [PubMed]
9 Mycoplasma pulmonis UAB CTIP
310
French CT, et al (2008). Large-scale transposon mutagenesis of Mycoplasma pulmonis. Mol Microbiol. [PubMed]
10 Pseudomonas aeruginosa UCBPP-PA14
335
Liberati NT, et al (2006). An ordered, nonredundant library of Pseudomonas aeruginosa strain PA14 transposon insertion mutants. Proc Natl Acad Sci U S A, 103:2833-8. [PubMed]
11 Salmonella typhimurium LT2
230
Knuth K, et al (2004). Large-scale identification of essential Salmonella genes by trapping lethal insertions. Mol Microbiol, 51:1729-44. [PubMed]
12 Staphylococcus aureus N315
302
Ji Y, et al (2001). Identification of critical staphylococcal genes using conditional phenotypes generated by antisense RNA. Science, 293:2266-9. [PubMed]

Forsyth RA, et al (2002). A genome-wide strategy for the identification of essential genes in Staphylococcus aureus. Mol Microbiol, 43:1387-400. [PubMed]

Ko KS, et al (2006). Screening of Essential Genes in Staphylococcus aureus N315 Using Comparative Genomics and Allelic Replacement Mutagenesis. J Microbiol Biotechnol, 16:623-32.
13 Streptococcus pneumoniae
244
Thanassi JA, et al (2002). Identification of 113 conserved essential genes using a high-throughput gene disruption system in Streptococcus pneumoniae. Nucleic Acids Res, 30:3152-62. [PubMed]

Song JH, et al (2005). Identification of Essential Genes in Streptococcus pneumoniae by Allelic Replacement Mutagenesis. Mol Cells, 19:365-74. [PubMed]
14 Vibrio cholerae
5
Judson N, Mekalanos JJ (2000). TnAraOut, a transposon-based approach to identify and characterize essential bacterial genes. Nat Biotechnol, 18:740-5. [PubMed]
No Organism (Eukaryotes) Essential genes Reference
1 Arabidopsis thaliana
777
Tzafrir I, et al (2004). Identification of genes required for embryo development in Arabidopsis. Plant Physiol, 135:1206-20. [PubMed]
2 Caenorhabditis elegans
294
Kamath RS, et al (2003). Systematic functional analysis of the Caenorhabditis elegans genome using RNAi. Nature, 421:231-7. [PubMed]
3 Danio rerio
288
Amsterdam A, et al (2004). Identification of 315 genes essential for early zebrafish development. Proc Natl Acad Sci U S A, 101:12792-7. [PubMed]
4 Drosophila melanogaster
339
Spradling AC, et al (1999). The Berkeley Drosophila Genome Project gene disruption project: Single P-element insertions mutating 25% of vital Drosophila genes. Genetics, 153:135-77. [PubMed]
5 Homo sapiens
118
Liao BY, Zhang J (2008). Null mutations in human and mouse orthologs frequently result in different phenotypes. Proc Natl Acad Sci U S A, 105:6987-92. [PubMed]
6 Mus musculus
2114
Liao BY, Zhang J (2007). Mouse duplicate genes are as essential as singletons. Trends Genet, 23:378-81. [PubMed]
7 Saccharomyces cerevisiae
1110
Giaever G, et al (2002). Functional profiling of the Saccharomyces cerevisiae genome. Nature, 418:387-91. [PubMed]

 

DEG 5.2 contains 10068 essential genes in the following Organisms (October 14, 2008).
No Organism (Prokaryotes) Essential genes Reference
1 Acinetobacter baylyi ADP1
499
de Berardinis V, et al (2008). A complete collection of single-gene deletion mutants of Acinetobacter baylyi ADP1. Mol Syst Biol. [PubMed]
2 Bacillus subtilis 168
271
Kobayashi K, et al (2003). Essential Bacillus subtilis genes. Proc Natl Acad Sci U S A, 100:4678-83. [PubMed]
3 Escherichia coli MG1655
712
Gerdes SY, et al (2003). Experimental determination and system level analysis of essential genes in Escherichia coli MG1655. J Bacteriol, 185:5673-84. [PubMed]

Baba T, et al (2006). Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol. [PubMed]
4 Francisella novicida U112
392
Gallagher LA, et al (2007). A comprehensive transposon mutant library of Francisella novicida, a bioweapon surrogate. Proc Natl Acad Sci U S A, 104:1009-14. [PubMed]
5 Haemophilus influenzae Rd KW20
642
Akerley BJ, et al (2002). A genome-scale analysis for identification of genes required for growth or survival of Haemophilus influenzae. Proc Natl Acad Sci U S A, 99:966-71. [PubMed]
6 Helicobacter pylori 26695
323
Salama NR, et al (2004). Global transposon mutagenesis and essential gene analysis of Helicobacter pylori. J Bacteriol, 186:7926-35. [PubMed]
7 Mycobacterium tuberculosis H37Rv
614
Sassetti CM, et al (2003). Genes required for mycobacterial growth defined by high density mutagenesis. Mol Microbiol, 48:77-84. [PubMed]
8 Mycoplasma genitalium G37
381
Glass JI, et al (2006). Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A, 103:425-30. [PubMed]
9 Mycoplasma pulmonis UAB CTIP
310
French CT, et al (2008). Large-scale transposon mutagenesis of Mycoplasma pulmonis. Mol Microbiol. [PubMed]
10 Pseudomonas aeruginosa UCBPP-PA14
335
Liberati NT, et al (2006). An ordered, nonredundant library of Pseudomonas aeruginosa strain PA14 transposon insertion mutants. Proc Natl Acad Sci U S A, 103:2833-8. [PubMed]
11 Salmonella typhimurium LT2
230
Knuth K, et al (2004). Large-scale identification of essential Salmonella genes by trapping lethal insertions. Mol Microbiol, 51:1729-44. [PubMed]
12 Staphylococcus aureus N315
302
Ji Y, et al (2001). Identification of critical staphylococcal genes using conditional phenotypes generated by antisense RNA. Science, 293:2266-9. [PubMed]

Forsyth RA, et al (2002). A genome-wide strategy for the identification of essential genes in Staphylococcus aureus. Mol Microbiol, 43:1387-400. [PubMed]

Ko KS, et al (2006). Screening of Essential Genes in Staphylococcus aureus N315 Using Comparative Genomics and Allelic Replacement Mutagenesis. J Microbiol Biotechnol, 16:623-32.
13 Streptococcus pneumoniae
244
Thanassi JA, et al (2002). Identification of 113 conserved essential genes using a high-throughput gene disruption system in Streptococcus pneumoniae. Nucleic Acids Res, 30:3152-62. [PubMed]

Song JH, et al (2005). Identification of Essential Genes in Streptococcus pneumoniae by Allelic Replacement Mutagenesis. Mol Cells, 19:365-74. [PubMed]
14 Vibrio cholerae
5
Judson N, Mekalanos JJ (2000). TnAraOut, a transposon-based approach to identify and characterize essential bacterial genes. Nat Biotechnol, 18:740-5. [PubMed]
No Organism (Eukaryotes) Essential genes Reference
1 Arabidopsis thaliana
777
Tzafrir I, et al (2004). Identification of genes required for embryo development in Arabidopsis. Plant Physiol, 135:1206-20. [PubMed]
2 Caenorhabditis elegans
294
Kamath RS, et al (2003). Systematic functional analysis of the Caenorhabditis elegans genome using RNAi. Nature, 421:231-7. [PubMed]
3 Danio rerio
288
Amsterdam A, et al (2004). Identification of 315 genes essential for early zebrafish development. Proc Natl Acad Sci U S A, 101:12792-7. [PubMed]
4 Drosophila melanogaster
339
Spradling AC, et al (1999). The Berkeley Drosophila Genome Project gene disruption project: Single P-element insertions mutating 25% of vital Drosophila genes. Genetics, 153:135-77. [PubMed]
5 Homo sapiens
118
Liao BY, Zhang J (2008). Null mutations in human and mouse orthologs frequently result in different phenotypes. Proc Natl Acad Sci U S A, 105:6987-92. [PubMed]
6 Mus musculus
2114
Liao BY, Zhang J (2007). Mouse duplicate genes are as essential as singletons. Trends Genet, 23:378-81. [PubMed]
7 Saccharomyces cerevisiae
878
http://mips.gsf.de/genre/proj/yeast/

 

DEG 5.0 contains 9948 essential genes in the following Organisms (June 12, 2008).
No Organism (Prokaryotes) Essential genes Reference
1 Acinetobacter baylyi ADP1
499
de Berardinis V, et al (2008). A complete collection of single-gene deletion mutants of Acinetobacter baylyi ADP1. Mol Syst Biol. [PubMed]
2 Bacillus subtilis 168
271
Kobayashi K, et al (2003). Essential Bacillus subtilis genes. Proc Natl Acad Sci U S A, 100:4678-83. [PubMed]
3 Escherichia coli MG1655
615
Gerdes SY, et al (2003). Experimental determination and system level analysis of essential genes in Escherichia coli MG1655. J Bacteriol, 185:5673-84. [PubMed]
4 Francisella novicida U112
392
Gallagher LA, et al (2007). A comprehensive transposon mutant library of Francisella novicida, a bioweapon surrogate. Proc Natl Acad Sci U S A, 104:1009-14. [PubMed]
5 Haemophilus influenzae Rd KW20
642
Akerley BJ, et al (2002). A genome-scale analysis for identification of genes required for growth or survival of Haemophilus influenzae. Proc Natl Acad Sci U S A, 99:966-71. [PubMed]
6 Helicobacter pylori 26695
323
Salama NR, et al (2004). Global transposon mutagenesis and essential gene analysis of Helicobacter pylori. J Bacteriol, 186:7926-35. [PubMed]
7 Helicobacter pylori J99
312
Salama NR, et al (2004). Global transposon mutagenesis and essential gene analysis of Helicobacter pylori. J Bacteriol, 186:7926-35. [PubMed]
8 Mycobacterium tuberculosis H37Rv
614
Sassetti CM, et al (2003). Genes required for mycobacterial growth defined by high density mutagenesis. Mol Microbiol, 48:77-84. [PubMed]
9 Mycoplasma genitalium G37
381
Glass JI, et al (2006). Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A, 103:425-30. [PubMed]
10 Mycoplasma pulmonis UAB CTIP
310
French CT, et al (2008). Large-scale transposon mutagenesis of Mycoplasma pulmonis. Mol Microbiol. [PubMed]
11 Salmonella typhimurium LT2
230
Knuth K, et al (2004). Large-scale identification of essential Salmonella genes by trapping lethal insertions. Mol Microbiol, 51:1729-44. [PubMed]
12 Staphylococcus aureus N315
302
Ji Y, et al (2001). Identification of critical staphylococcal genes using conditional phenotypes generated by antisense RNA. Science, 293:2266-9. [PubMed]

Forsyth RA, et al (2002). A genome-wide strategy for the identification of essential genes in Staphylococcus aureus. Mol Microbiol, 43:1387-400. [PubMed]

Ko KS, et al (2006). Screening of Essential Genes in Staphylococcus aureus N315 Using Comparative Genomics and Allelic Replacement Mutagenesis. J Microbiol Biotechnol, 16:623-32.
13 Streptococcus pneumoniae
244
Thanassi JA, et al (2002). Identification of 113 conserved essential genes using a high-throughput gene disruption system in Streptococcus pneumoniae. Nucleic Acids Res, 30:3152-62. [PubMed]

Song JH, et al (2005). Identification of Essential Genes in Streptococcus pneumoniae by Allelic Replacement Mutagenesis. Mol Cells, 19:365-74. [PubMed]
14 Vibrio cholerae
5
Judson N, Mekalanos JJ (2000). TnAraOut, a transposon-based approach to identify and characterize essential bacterial genes. Nat Biotechnol, 18:740-5. [PubMed]
No Organism (Eukaryotes) Essential genes Reference
1 Arabidopsis thaliana
777
Tzafrir I, et al (2004). Identification of genes required for embryo development in Arabidopsis. Plant Physiol, 135:1206-20. [PubMed]
2 Caenorhabditis elegans
294
Kamath RS, et al (2003). Systematic functional analysis of the Caenorhabditis elegans genome using RNAi. Nature, 421:231-7. [PubMed]
3 Danio rerio
288
Amsterdam A, et al (2004). Identification of 315 genes essential for early zebrafish development. Proc Natl Acad Sci U S A, 101:12792-7. [PubMed]
4 Drosophila melanogaster
339
Spradling AC, et al (1999). The Berkeley Drosophila Genome Project gene disruption project: Single P-element insertions mutating 25% of vital Drosophila genes. Genetics, 153:135-77. [PubMed]
5 Homo sapiens
118
Liao BY, Zhang J (2008). Null mutations in human and mouse orthologs frequently result in different phenotypes. Proc Natl Acad Sci U S A, 105:6987-92. [PubMed]
6 Mus musculus
2114
Liao BY, Zhang J (2007). Mouse duplicate genes are as essential as singletons. Trends Genet, 23:378-81. [PubMed]
7 Saccharomyces cerevisiae
878
http://mips.gsf.de/genre/proj/yeast/

 

DEG 4.1 contains 5140 essential genes in the following Organisms (June 2, 2008).
No Organism Essential genes Reference
1 Acinetobacter baylyi ADP1
499
de Berardinis V, et al (2008). A complete collection of single-gene deletion mutants of Acinetobacter baylyi ADP1. Mol Syst Biol. [PubMed]
2 Bacillus subtilis 168
271
Kobayashi K, et al (2003). Essential Bacillus subtilis genes. Proc Natl Acad Sci U S A, 100:4678-83. [PubMed]
3 Escherichia coli MG1655
615
Gerdes SY, et al (2003). Experimental determination and system level analysis of essential genes in Escherichia coli MG1655. J Bacteriol, 185:5673-84. [PubMed]
4 Francisella novicida U112
392
Gallagher LA, et al (2007). A comprehensive transposon mutant library of Francisella novicida, a bioweapon surrogate. Proc Natl Acad Sci U S A, 104:1009-14. [PubMed]
5 Haemophilus influenzae Rd KW20
642
Akerley BJ, et al (2002). A genome-scale analysis for identification of genes required for growth or survival of Haemophilus influenzae. Proc Natl Acad Sci U S A, 99:966-71. [PubMed]
6 Helicobacter pylori 26695
323
Salama NR, et al (2004). Global transposon mutagenesis and essential gene analysis of Helicobacter pylori. J Bacteriol, 186:7926-35. [PubMed]
7 Helicobacter pylori J99
312
Salama NR, et al (2004). Global transposon mutagenesis and essential gene analysis of Helicobacter pylori. J Bacteriol, 186:7926-35. [PubMed]
8 Mycobacterium tuberculosis H37Rv
614
Sassetti CM, et al (2003). Genes required for mycobacterial growth defined by high density mutagenesis. Mol Microbiol, 48:77-84. [PubMed]
9 Mycoplasma genitalium G37
381
Glass JI, et al (2006). Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A, 103:425-30. [PubMed]
10 Mycoplasma pulmonis UAB CTIP
310
French CT, et al (2008). Large-scale transposon mutagenesis of Mycoplasma pulmonis. Mol Microbiol. [PubMed]
11 Salmonella typhimurium LT2
230
Knuth K, et al (2004). Large-scale identification of essential Salmonella genes by trapping lethal insertions. Mol Microbiol, 51:1729-44. [PubMed]
12 Staphylococcus aureus N315
302

Ji Y, et al (2001). Identification of critical staphylococcal genes using conditional phenotypes generated by antisense RNA. Science, 293:2266-9. [PubMed]

Forsyth RA, et al (2002). A genome-wide strategy for the identification of essential genes in Staphylococcus aureus. Mol Microbiol, 43:1387-400. [PubMed]

Ko KS, et al (2006). Screening of Essential Genes in Staphylococcus aureus N315 Using Comparative Genomics and Allelic Replacement Mutagenesis. J Microbiol Biotechnol, 16:623-32. [PubMed]

13 Streptococcus pneumoniae
244

Thanassi JA, et al (2002). Identification of 113 conserved essential genes using a high-throughput gene disruption system in Streptococcus pneumoniae. Nucleic Acids Res, 30:3152-62. [PubMed]

Song JH, et al (2005). Identification of Essential Genes in Streptococcus pneumoniae by Allelic Replacement Mutagenesis. Mol Cells, 19:365-74. [PubMed]

14 Vibrio cholerae
5
Judson N, Mekalanos JJ (2000). TnAraOut, a transposon-based approach to identify and characterize essential bacterial genes. Nat Biotechnol, 18:740-5. [PubMed]

 

DEG 4.0 contains 4624 essential genes in the following Organisms (May 19, 2008).
No Organism Essential genes Reference
1 Acinetobacter baylyi ADP1
293
de Berardinis V, et al (2008). A complete collection of single-gene deletion mutants of Acinetobacter baylyi ADP1. Mol Syst Biol. [PubMed]
2 Bacillus subtilis 168
271
Kobayashi K, et al (2003). Essential Bacillus subtilis genes. Proc Natl Acad Sci U S A, 100:4678-83. [PubMed]
3 Escherichia coli MG1655
615
Gerdes SY, et al (2003). Experimental determination and system level analysis of essential genes in Escherichia coli MG1655. J Bacteriol, 185:5673-84. [PubMed]
4 Francisella novicida U112
392
Gallagher LA, et al (2007). A comprehensive transposon mutant library of Francisella novicida, a bioweapon surrogate. Proc Natl Acad Sci U S A, 104:1009-14. [PubMed]
5 Haemophilus influenzae Rd KW20
642
Akerley BJ, et al (2002). A genome-scale analysis for identification of genes required for growth or survival of Haemophilus influenzae. Proc Natl Acad Sci U S A, 99:966-71. [PubMed]
6 Helicobacter pylori 26695
323
Salama NR, et al (2004). Global transposon mutagenesis and essential gene analysis of Helicobacter pylori. J Bacteriol, 186:7926-35. [PubMed]
7 Helicobacter pylori J99
312
Salama NR, et al (2004). Global transposon mutagenesis and essential gene analysis of Helicobacter pylori. J Bacteriol, 186:7926-35. [PubMed]
8 Mycobacterium tuberculosis H37Rv
614
Sassetti CM, et al (2003). Genes required for mycobacterial growth defined by high density mutagenesis. Mol Microbiol, 48:77-84. [PubMed]
9 Mycoplasma genitalium G37
381
Glass JI, et al (2006). Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A, 103:425-30. [PubMed]
10 Salmonella typhimurium LT2
230
Knuth K, et al (2004). Large-scale identification of essential Salmonella genes by trapping lethal insertions. Mol Microbiol, 51:1729-44. [PubMed]
11 Staphylococcus aureus N315
302

Ji Y, et al (2001). Identification of critical staphylococcal genes using conditional phenotypes generated by antisense RNA. Science, 293:2266-9. [PubMed]

Forsyth RA, et al (2002). A genome-wide strategy for the identification of essential genes in Staphylococcus aureus. Mol Microbiol, 43:1387-400. [PubMed]

Ko KS, et al (2006). Screening of Essential Genes in Staphylococcus aureus N315 Using Comparative Genomics and Allelic Replacement Mutagenesis. J Microbiol Biotechnol, 16:623-32. [PubMed]

12 Streptococcus pneumoniae
244

Thanassi JA, et al (2002). Identification of 113 conserved essential genes using a high-throughput gene disruption system in Streptococcus pneumoniae. Nucleic Acids Res, 30:3152-62. [PubMed]

Song JH, et al (2005). Identification of Essential Genes in Streptococcus pneumoniae by Allelic Replacement Mutagenesis. Mol Cells, 19:365-74. [PubMed]

13 Vibrio cholerae
5
Judson N, Mekalanos JJ (2000). TnAraOut, a transposon-based approach to identify and characterize essential bacterial genes. Nat Biotechnol, 18:740-5. [PubMed]

 

DEG 3.2 contains 4528 essential genes in the following Organisms (February 9, 2007).
No Organism Essential genes Reference
1 Bacillus subtilis
248
Kobayashi, K. et al., 2003 Essential Bacillus subtilis genes. Proc Natl Acad Sci U S A 100: 4678-4683. [PubMed]
2 Escherichia coli MG1655
619
Gerdes, S.Y. et al., 2003 Experimental determination and system level analysis of essential genes in Escherichia coli MG1655. J Bacteriol. 185(19):5673-84. [PubMed]
3 Haemophilus influenzae
638
Akerley, B.J. et al., 2002 A genome-scale analysis for identification of genes required for growth or survival of Haemophilus influenzae. Proc Natl Acad Sci U S A 99: 966-71. [PubMed]
4 Helicobacter pylori
343
Salama, N.R. et al.,2004 Global transposon mutagenesis and essential gene analysis of Helicobacter pylori. J Bacteriol.186: 7926-7935. [PubMed]
5 Mycobacterium tuberculosis H37Rv
614
Sassetti, C.M. et al., 2003 Genes required for mycobacterial growth defined by high density mutagenesis. Mol Microbiol. 48(1):77-84. [PubMed]
6 Mycoplasma genitalium
381
Hutchison, C.A. et al., 1999 Global transposon mutagenesis and a minimal Mycoplasma genome. Science. 286:2165-2169. [PubMed]

Glass, J.I. et al., 2006 Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103: 425-30. [PubMed]
7 Saccharomyces cerevisiae
878
http://www.mips.biochem.mpg.de/proj/yeast
8 Salmonella typhimurium
251
Knuth, K. et al., 2004 Large-scale identification of essential Salmonella genes by trapping lethal insertions. Mol Microbiol. 51(6):1729-44. [PubMed]
9 Staphylococcus aureus
308

Ji, Y. et al., 2001 Identification of critical staphylococcal genes using conditional phenotypes generated by antisense RNA. Science 293: 2266-2269. [PubMed]

Forsyth, R.A. et al., 2002 A genome-wide strategy for the identification of essential genes in Staphylococcus aureus. Mol Microbiol. 43:1387-400. [PubMed]

Ko, K.S. et al., 2006 Screening of Essential Genes in Staphylococcus aureus N315 Using Comparative Genomics and Allelic Replacement Mutagenesis. J. Microbiol. Biotechnol. 16(4):623-632. [PubMed]

10 Streptococcus pneumoniae
243

Thanassi, J.A. et al., 2002 Identification of 113 conserved essential genes using a high-throughput gene disruption system in Streptococcus pneumoniae. Nucleic Acids Res. 30:3152-62. [PubMed]

Song, J.H. et al., 2005 Identification of Essential Genes in Streptococcus pneumoniae by Allelic Replacement Mutagenesis. Mol Cells. 19(3):365-74. [PubMed]

11 Vibrio cholerae
5
Judson, N., and J. J. Mekalanos, 2000 TnAraOut, a transposon-based approach to identify and characterize essential bacterial genes. Nat Biotechnol 18: 740-745. [PubMed]

 

DEG 3.0 contains 4273 essential genes in the following Organisms (October 23, 2006).
No Organism Essential genes Reference
1 Bacillus subtilis
248
Kobayashi, K. et al., 2003 Essential Bacillus subtilis genes. Proc Natl Acad Sci U S A 100: 4678-4683. [PubMed]
2 Escherichia coli MG1655
619
Gerdes, S.Y. et al., 2003 Experimental determination and system level analysis of essential genes in Escherichia coli MG1655. J Bacteriol. 185(19):5673-84. [PubMed]
3 Haemophilus influenzae
638
Akerley, B.J. et al., 2002 A genome-scale analysis for identification of genes required for growth or survival of Haemophilus influenzae. Proc Natl Acad Sci U S A 99: 966-71. [PubMed]
4 Helicobacter pylori
343
Salama, N.R. et al.,2004 Global transposon mutagenesis and essential gene analysis of Helicobacter pylori. J Bacteriol.186: 7926-7935. [PubMed]
5 Mycobacterium tuberculosis H37Rv
614
Sassetti, C.M. et al., 2003 Genes required for mycobacterial growth defined by high density mutagenesis. Mol Microbiol. 48(1):77-84. [PubMed]
6 Mycoplasma genitalium
381
Hutchison, C.A. et al., 1999 Global transposon mutagenesis and a minimal Mycoplasma genome. Science. 286:2165-2169. [PubMed]

Glass, J.I. et al., 2006 Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103: 425-30. [PubMed]
7 Saccharomyces cerevisiae
878
http://www.mips.biochem.mpg.de/proj/yeast
8 Salmonella typhimurium
251
Knuth, K. et al., 2004 Large-scale identification of essential Salmonella genes by trapping lethal insertions. Mol Microbiol. 51(6):1729-44. [PubMed]
9 Staphylococcus aureus
186

Ji, Y. et al., 2001 Identification of critical staphylococcal genes using conditional phenotypes generated by antisense RNA. Science 293: 2266-2269. [PubMed]

Forsyth, R.A. et al., 2002 A genome-wide strategy for the identification of essential genes in Staphylococcus aureus. Mol Microbiol. 43:1387-400. [PubMed]

10 Streptococcus pneumoniae Rx-1
110
Thanassi, J.A. et al., 2002 Identification of 113 conserved essential genes using a high-throughput gene disruption system in Streptococcus pneumoniae. Nucleic Acids Res. 30:3152-62. [PubMed]
11 Vibrio cholerae
5
Judson, N., and J. J. Mekalanos, 2000 TnAraOut, a transposon-based approach to identify and characterize essential bacterial genes. Nat Biotechnol 18: 740-745. [PubMed]

 

DEG 2.5 contains 3024 essential genes in the following Organisms (April 23, 2006).
No Organism Essential genes Reference
1 Bacillus subtilis
248
Kobayashi, K. et al., 2003 Essential Bacillus subtilis genes. Proc Natl Acad Sci U S A 100: 4678-4683. [PubMed]
2 Escherichia coli
235
http://www.shigen.nig.ac.jp/ecoli/pec/index.jsp
3 Haemophilus influenzae
638
Akerley, B.J. et al., 2002 A genome-scale analysis for identification of genes required for growth or survival of Haemophilus influenzae. Proc Natl Acad Sci U S A 99: 966-71. [PubMed]
4 Helicobacter pylori
343
Salama, N.R. et al.,2004 Global transposon mutagenesis and essential gene analysis of Helicobacter pylori. J Bacteriol.186: 7926-7935. [PubMed]
5 Mycoplasma genitalium
381
Hutchison, C.A. et al., 1999 Global transposon mutagenesis and a minimal Mycoplasma genome. Science. 286:2165-2169. [PubMed]

Glass, J.I. et al., 2006 Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103: 425-30. [PubMed]
6 Saccharomyces cerevisiae
878
http://www.mips.biochem.mpg.de/proj/yeast
7 Staphylococcus aureus
186

Ji, Y. et al., 2001 Identification of critical staphylococcal genes using conditional phenotypes generated by antisense RNA. Science 293: 2266-2269. [PubMed]

Forsyth, R.A. et al., 2002 A genome-wide strategy for the identification of essential genes in Staphylococcus aureus. Mol Microbiol. 43:1387-400. [PubMed]

8 Streptococcus pneumoniae Rx-1
110
Thanassi, J.A. et al., 2002 Identification of 113 conserved essential genes using a high-throughput gene disruption system in Streptococcus pneumoniae. Nucleic Acids Res. 30:3152-62. [PubMed]
9 Vibrio cholerae
5
Judson, N., and J. J. Mekalanos, 2000 TnAraOut, a transposon-based approach to identify and characterize essential bacterial genes. Nat Biotechnol 18: 740-745. [PubMed]

 

DEG 2.0 contains 2755 essential genes in the following Organisms (March 23, 2005).
No Organism Essential genes Reference
1 Bacillus subtilis
248
Kobayashi, K. et al., 2003 Essential Bacillus subtilis genes. Proc Natl Acad Sci U S A 100: 4678-4683. [PubMed]
2 Escherichia coli
235
http://www.shigen.nig.ac.jp/ecoli/pec/index.jsp
3 Haemophilus influenzae
638
Akerley, B.J. et al., 2002 A genome-scale analysis for identification of genes required for growth or survival of Haemophilus influenzae. Proc Natl Acad Sci U S A 99: 966-71. [PubMed]
4 Helicobacter pylori
326
Salama, N.R. et al.,2004 Global transposon mutagenesis and essential gene analysis of Helicobacter pylori. J Bacteriol.186: 7926-7935. [PubMed]
5 Mycoplasma genitalium
129
Hutchison, C.A. et al., 1999 Global transposon mutagenesis and a minimal Mycoplasma genome. Science. 286:2165-2169. [PubMed]
6 Saccharomyces cerevisiae
878
http://www.mips.biochem.mpg.de/proj/yeast
7 Staphylococcus aureus
186

Ji, Y. et al., 2001 Identification of critical staphylococcal genes using conditional phenotypes generated by antisense RNA. Science 293: 2266-2269. [PubMed]

Forsyth, R.A. et al., 2002 A genome-wide strategy for the identification of essential genes in Staphylococcus aureus. Mol Microbiol. 43:1387-400. [PubMed]

8 Streptococcus pneumoniae Rx-1
110
Thanassi, J.A. et al., 2002 Identification of 113 conserved essential genes using a high-throughput gene disruption system in Streptococcus pneumoniae. Nucleic Acids Res. 30:3152-62. [PubMed]
9 Vibrio cholerae
5
Judson, N., and J. J. Mekalanos, 2000 TnAraOut, a transposon-based approach to identify and characterize essential bacterial genes. Nat Biotechnol 18: 740-745. [PubMed]

 

DEG 1.1 contains 2248 essential genes in the following Organisms (December 29, 2003).
No Organism Essential genes Reference
1 Bacillus subtilis
248
Kobayashi, K. et al., 2003 Essential Bacillus subtilis genes. Proc Natl Acad Sci U S A 100: 4678-4683. [PubMed]
2 Escherichia coli
54
http://magpie.genome.wisc.edu/~chris/essential.html
3 Haemophilus influenzae
638
Akerley, B.J. et al., 2002 A genome-scale analysis for identification of genes required for growth or survival of Haemophilus influenzae. Proc Natl Acad Sci U S A 99: 966-71. [PubMed]
4 Mycoplasma genitalium
129
Hutchison, C.A. et al., 1999 Global transposon mutagenesis and a minimal Mycoplasma genome. Science. 286:2165-2169. [PubMed]
5 Saccharomyces cerevisiae
878
http://www.mips.biochem.mpg.de/proj/yeast
6 Staphylococcus aureus
186
Ji, Y. et al., 2001 Identification of critical staphylococcal genes using conditional phenotypes generated by antisense RNA. Science 293: 2266-2269. [PubMed]

Forsyth R.A. et al., 2002 A genome-wide strategy for the identification of essential genes in Staphylococcus aureus. Mol Microbiol. 43:1387-400. [PubMed]
7 Streptococcus pneumoniae Rx-1
110
Thanassi, J.A. et al., 2002 Identification of 113 conserved essential genes using a high-throughput gene disruption system in Streptococcus pneumoniae. Nucleic Acids Res. 30:3152-62. [PubMed]
8 Vibrio cholerae
5
Judson, N., and J. J. Mekalanos, 2000 TnAraOut, a transposon-based approach to identify and characterize essential bacterial genes. Nat Biotechnol 18: 740-745. [PubMed]

 

DEG 1.0 contains 1421 essential genes in the following Organisms (October 21, 2002).
No Organism Essential genes Reference
1 Bacillus subtilis
224
Kobayashi, K. et al., 2003 Essential Bacillus subtilis genes. Proc Natl Acad Sci U S A 100: 4678-4683. [PubMed]
2 Escherichia coli
54
http://magpie.genome.wisc.edu/~chris/essential.html
3 Haemophilus influenzae
109
Mushegian, A. R., and E. V. Koonin, 1996 A minimal gene set for cellular life derived by comparison of complete bacterial genomes. Proc Natl Acad Sci U S A 93: 10268-10273. [PubMed]
4 Mycoplasma genitalium
86
Mushegian, A. R., and E. V. Koonin, 1996 A minimal gene set for cellular life derived by comparison of complete bacterial genomes. Proc Natl Acad Sci U S A 93: 10268-10273. [PubMed]
5 Saccharomyces cerevisiae
878
http://www.mips.biochem.mpg.de/proj/yeast
6 Staphylococcus aureus
65
Ji, Y. et al., 2001 Identification of critical staphylococcal genes using conditional phenotypes generated by antisense RNA. Science 293: 2266-2269. [PubMed]
7 Vibrio cholerae
5
Judson, N., and J. J. Mekalanos, 2000 TnAraOut, a transposon-based approach to identify and characterize essential bacterial genes. Nat Biotechnol 18: 740-745. [PubMed]
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