Complete and accurate DNA replication is fundamental progress for all organisms, and this event initiates at origins of replication. The characteristics of eukaryotic replication origins are best understood in Saccharomyces cerevisiae. Here, we present a novel pipeline for the computational prediction of replication origin sequences in the genome-wide level based only on pure genome sequences, and we also actively developed a new predictor called Ori-Finder 3.
The ARSs prediction pipeline, Ori-Finder 3, can predict the potential ARSs among the sequences with any length greater than 50 bp at the same time, which makes it possible for researchers to efficiently locate the ARSs from the yeast genomes utilizing the bioinformatic method. Based on the Ori-Finder 3, we subsequently build a database of predicted ARSs among a hundred of S. cerevisiae genomics. This prediction pipeline provides a powerful way for effectively predicting the potential ARSs on the genome-wide level when only the DNA sequence is available.